How are you targeting the protein to the membrane and to which one?
Which strain of E coli are you using? BL21(De3) may not be the best. Especially
if the gene is not codon optimized. You could try Rosetta cells. Furthermore,
you may get better expression with the Walker Strains (C41 and C43)
Dear Clement
DIALS is one of the packages which supports reading of and processing of Eiger
HDF5 data, and is available from
http://dials.diamond.ac.uk/
(dials.image_viewer is the tool you want here)
Any problems please don’t hesitate to contact
