Re: [ccp4bb] iMosflm twist large variance and specific space group scale output

Dr. Harry, Thank you very much for the illustration! On Wed, Feb 15, 2017 at 2:07 PM, Harry Powell wrote: > Hi > > First things first... > > 2. I am not sure if iMosflm can be set to output the scale of specific > space group. For example, in my data set even though

Re: [ccp4bb] iMosflm twist large variance and specific space group scale output

Hi First things first... > 2. I am not sure if iMosflm can be set to output the scale of specific space > group. For example, in my data set even though the integration is done at > P321 space group, after click "quick symm" or "quick scale" it seems the > program automatically choose what it

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

Well GE sells empty columns (XK for low back-pressure, tricorn for higher back-pressure) that you can fill with the resin you want. They seem expensive but for that kind of things I don't think any option will be real 'savings'. For nickel affinity we have been having good results with Roche's

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

Hi Markus, BioRad has columns as well (prepacked and empty), which follow the establiched connector standard. So usable without the need of adapters. Cheers Christian Am 15.02.2017 um 16:57 schrieb Markus Seeliger: Dear all, we are happy users of all that GE offers around their FPLC

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

You could try Goldbio.com. I have not used the His-columns from them, but have used the Protein A columns and they work as well as the Protein A from GE. Daniel A Bonsor PhD. Sundberg Lab Institute of Human Virology University of Maryland, Baltimore 725 W Lombard Street N370 Baltimore Maryland

[ccp4bb] off topic: Alternatives to GE HiTrap columns

Dear all, we are happy users of all that GE offers around their FPLC system, but I am getting a little tired of feeling monopolized. Are any of you aware of either empty columns or prepacked columns (e.g. metal affinity or ion exchange resins) from other companies? Thanks for your advice Markus

[ccp4bb] iMosflm twist large variance and specific space group scale output

Dear CCP4bb members, I have two questions about imosflm integration and scaling process (my data is 3A resolution and 0.5 degree mosaicity): 1. I have warnings after integration saying the twist variance is too large (from -0.26 to 0.33, which is larger than 0.5), suggesting that cell parameters

[ccp4bb] Off topic: Fellowship Day Department of Chemistry University of York/YSBL

Dear all, on behalf of the Department of Chemistry, to which the York Structural Biology Lab (YSBL) belongs, I post this notice about the Fellowship Open Day. If you are interested to work in York in YSBL as a Fellow please register, come to York and find out how the Department supports you

[ccp4bb] EMBO practical course on image processing for cryo-electron microscopy

Posted on behalf of Elena Orlova... Dear All, It is a pleasure to announce that we are running the next EMBO practical course on image processing for cryo-electron microscopy in Birkbeck College , ISMB, London . This 10 day course will be held in September (5 - 15), 2017. You can find our