Re: [ccp4bb] High Rfree: Phasing issue or partial crystal disorder

2017-04-19 Thread James Holton
In situations like this I always try dropping to P1. Even if the data are highly incomplete in P1 you can still refine it. Difference maps are degraded by poor completeness, but you still might see something. But either way, the R-factors will tell you something. if dropping to P1 solves

Re: [ccp4bb] Pymol point mutagenesis turns out to be deletion of a residue

2017-04-19 Thread Alex Lee
thanks. Coot works! On Wed, Apr 19, 2017 at 10:20 AM, Vipul Panchal wrote: > I am not experienced with pymol. However if you are familiarized with > coot, you can mutate and set rotamer. It is really simple. > > On 12-Apr-2017 12:15 AM, "Alex Lee"

[ccp4bb] ADSC Q4 systems available

2017-04-19 Thread Henry D Bellamy
We have an ADSC Q4 system and a Q4R system that we no longer need.The detectors have not been used for several years and have probably lost vacuum and therefore need recalibration. In the likely event that no-one wants the entire system we will split it up for those who want parts such

[ccp4bb] ISBC2017 Granada

2017-04-19 Thread Jose A. Gavira
Just a quick remainder. Last Five days for the low-rate registration at the 6th International School on Biological Crystallization (ISBC2017). May 29th to Jun 02nd. For more information, please visit http://www.isbcgranada.org/. Best regards, ISBC 2017

[ccp4bb] Antw: Re: [ccp4bb] When should one add solvents

2017-04-19 Thread Matthias Barone
Hi Abhishek Im by far not as experienced as others in the bulletin board, but I made the experience that automated water update with Phenix leads to an aggressive placement of solvent molecules, which can be damped a bit by restricting H-bond distances of newly placed waters. As Robbie and