[ccp4bb] Computer scientist position - MPI Dortmund

2018-05-13 Thread Stefan Raunser
Dear colleagues, I would like to draw your attention to the following job opportunity. Best Stefan Computer scientist position at Max Planck Institute of Molecular Physiology Job description: Our research focuses on the structural analysis of macromolecular protein complexes using single-part

Re: [ccp4bb] Electron density

2018-05-13 Thread Keller, Jacob
I know you mentioned trying buffer components, but it does look a lot like TRIS to me, maybe a different conformation than you’re modelling? JPK + Jacob Pearson Keller Research Scientist / Looger Lab HHMI Janelia Research Campus 19700 Helix Dr, Ash

Re: [ccp4bb] Electron density

2018-05-13 Thread Daniel Garcia
Thanks for your quick responses. I used ethylene glycol as cryoprotectant. Indeed I had 1% DMSO as part of the crystallisation condition (I forgot to include that), but DMSO is too small to fit this density and DMSO has a trigonal pyramidal structure, which differs a bit to what I have in my struct

Re: [ccp4bb] Electron density

2018-05-13 Thread Ethan Merritt
On Monday, 14 May 2018 10:39:32 Daniel Garcia wrote: > Dear all, > > I am currently refining a structure and found a intriguing electron density > at the protein surface (pictures attached, the Fo-Fc map is contoured at > >3.5 sigma). My first candidates were molecules from my protein prep or > cr

Re: [ccp4bb] Electron density

2018-05-13 Thread Joel Tyndall
Did you happen to use glycerol as a cryoprotectant? From: CCP4 bulletin board On Behalf Of Daniel Garcia Sent: Monday, 14 May 2018 12:40 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Electron density Dear all, I am currently refining a structure and found a intriguing electron density at the

[ccp4bb] Postdoctoral scientist position in Structural Biology / Biophysics: Dundee, UK

2018-05-13 Thread Dr Alessio Ciulli
Dear all We are currently looking for a highly motivated structural biologist to join a multi-disciplinary team of drug discovery scientists. This team is engaged in an exciting drug discovery collaboration between my laboratory and Boehringer Ingelheim. Our mission is to discover and develop a