Re: [ccp4bb] PhD opportunity on Mycobacterial structural biology at Monash University, Melbourne

[SR Bharath]

zl...@imcb.a-star.edu.sg
On Sat, 21 Jul 2018 at 19:57, Rhys Grinter <
22087c81e8c6-dmarc-requ...@jiscmail.ac.uk> wrote:

> Dear All,
>
> There is an exciting opportunity to do a PhD co-supervised by Dr. Chris
> Greening and myself at Monash University, Melbourne. The project will
> investigate the structural basis for persistence in Mycobacterium
> tuberculosis, through the structural characterisation of the F420
> biosynthesis pathway. This multidisciplinary project will combine
> structural techniques (crystallography, cryo-EM), with biophysics,
> biochemistry and microbiology.
> https://www.findaphd.com/search/ProjectDetails.aspx?PJID=74941
>
> As well as being situated in Melbourne the worlds most liveable city,
> Monash University is within walking distance of the Australian Synchrotron
> and is home to a cutting edge Cryo-EM microscope facility. Making it a
> great place to do structural biology.
>
> Interested candidates should send their CV, academic transcript, and a
> brief outline of research interests and motivation to
> chris.green...@monash.edu.
>
> Please share with your colleagues and anyone interesting in starting a PhD
> in this exciting area.
>
> Best Wishes,
>
> Rhys
>
> --
> Dr Rhys Grinter
> Sir Henry Wellcome Fellow
> Monash University
> +61 (0)3 9902 9213
> +61 (0)403 896 767
>
> --
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
>



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] Postdoctoral Position in Structural Biology at The University of Oklahoma

[Nate Lavey]

Hi All,

I'm posting this on behalf of my PI Dr. Adam S. Duerfeldt, please see below. 
Thanks!

The Duerfeldt Lab (http://www.duerfeldtlabs.com) in the Department of Chemistry 
& Biochemistry at the University of Oklahoma has an immediate opening for a 
highly-motivated postdoc trained in microbiology, molecular biology, and/or 
structural biology. The major research interest of the lab is to exploit 
bacterial enzyme/pathway activation as a strategy to diminish microbial fitness 
and/or pathogenicity. The ideal applicant should have an interest in 
determining the structure and function of specified proteins within pathogenic 
organisms to uncover the therapeutic potential of these targets. We are located 
on the same floor as the University of Oklahoma’s Structural Biology Center of 
Biomedical Research Excellence (COBRE) and as a COBRE affiliated lab, the 
Duerfeldt Group has prioritized access to all equipment, facilities, and 
expertise in the COBRE cores. More information about the cores can be found at 
the structuralbiology.ou.edu/. This represents a great opportunity for a 
postdoc trained in the aforementioned areas to contribute complementary 
expertise to the major research initiatives of the group, all while being 
immersed in a medicinal chemistry focused environment.

Start Date: as soon as possible

To apply, please send a CV, research summary, and contact information for 3 
references to adam.duerfe...@ou.edu. Only complete applications will be 
reviewed.



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] Senior Postdoctoral Position on Cryo-EM in the Dep. for Protein Evolution at the MPI for Dev. Biology

[Joana Pereira]

Dear CCP4 colleagues,Our department is looking for a senior postdoc with experience on Cryo-EM to provide
the department with cutting-edge competence in protein structure determination with this method. The position is suitable to start an independent group and independent publication and grant
acquisition are encouraged. Please find more information on the attached file :) The deadline for application is the 15th of September 2018.All the best,Dr. Joana PereiraPostdoctoral Researcher Department of Protein Evolution Max Planck Institute for Developmental Biology Max-Planck-Ring 572076 Tübingen GERMANY

To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

<>
Begin forwarded message:From: "Karin Lehmann" Subject: Job offer for a Postdoc positionDate: 25. July 2018 at 10:54:57 CESTTo: Dear all,Please find attached a job advertisement for a Senior Postdoctoral Position in Protein Structure Determination.Best,Karin*Karin LehmannAssistant to Prof. Dr. Andrei LupasMax Planck Institute for Developmental BiologyMax-Planck-Ring 572076 Tuebingen/GermanyTel. +49-(0)7071-601340 Fax  +49-(0)7071-601352 e-mail: karin.lehm...@tuebingen.mpg.de

To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] Postdoctoral position on novel method for protein crystallisation

[Ioannis Vakonakis]

Dear colleagues,

A postdoctoral position is available immediately in my group jointly with the 
lab of Maike Bublitz, in the Department of Biochemistry, Oxford. The aim of 
this BBSRC-funded post is to develop a novel methodology for membrane protein 
crystallisation that utilises NMR-derived information and very high magnetic 
fields. Candidates with experience in membrane protein crystallisation and/or 
NMR are particularly encouraged to apply. Due to limitations from the funder 
the post holder needs to start by December 1st.

Further information on this post, and instructions on how to apply, are 
available here:
https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_details_form.jobspec?p_id=136086
The application deadline is Aug. 31st and interviews of shortlisted candidates 
will be held shortly thereafter.

Sincerely,

John Vakonakis







To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] O-linked glycans

[Thomas Lütteke]

Dear Goran et al.,

when building O-linked glycans one should keep in mind that in contrast to 
N-glycans there is no single core structure in O-glycans, but a variety of 
different structures exist. Therefore, the NAG-SER linkage might be correct (if 
it is an O-GlcNAc modification, which is usually used as a molecular switch in 
a similar way as phosphorylation, see 
https://www.ncbi.nlm.nih.gov/books/NBK453063/ ), but it might be incorrect if 
it is a different kind of O-glycan, such as mucin-type O-glycan (starting with 
A2G, see https://www.ncbi.nlm.nih.gov/books/NBK453030/ ), O-mannosylation 
(starting with MAN, see e.g. 
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500819/ ), a proteoglycan linker 
(starting with XYP, see https://www.ncbi.nlm.nih.gov/books/NBK453033/ ), or 
even one of some rare types of O-glycans (see 
https://www.ncbi.nlm.nih.gov/books/NBK453017/ ). This list might not be 
complete, as O-glycans are not my core expertise.
It is probably not possible to identify the O-glycan type in the electron 
density, but it might help to check the literature if something is known on the 
type of O-glycan at that position of your protein, so that you can model the 
correct residue, or at least the most likely one, into your electron density.

Best regards,
Thomas



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

[ccp4bb] Call for MX beamtime proposals at HZB, BESSY II, deadline September 01, 2018

[Manfred S. Weiss]

Dear all,

the next MX-proposal application deadline: September 01, 2018 is approaching
https://www.helmholtz-berlin.de/user/beamtime/proposals/index_en.html

This time all proposals will be handled with the new version of GATE,
GATE2.0
https://www.helmholtz-berlin.de/pubbin/hzbgate

Hereby we would like to invite the submission of new proposals for
MX-beamtime at the HZB-MX beamlines for the next beam time period
(02/2019-08/2019).

In order to apply for beamtime, please register at the HZB on-line
access tool "GATE" (https://www.helmholtz-berlin.de/pubbin/hzbgate)
and submit a new beam time application proposal.

With the new GATE version, the proposal categories have been
improved. We now expect from each group only ONE BAG proposal.
Each such proposals can contain up to 20 individual projects.

HZB provides MX-beamtime at the three MX-beamlines BL14.1, BL14.2
and BL14.3. The three beamlines are equipped with state-of-the-art
instrumentation and are currently the most productive MX-stations
in Germany with over 300 PDB depositions annually. Beamtime is
granted based on the reviewed proposals and on reports from
previous research activities. Please make sure to include them
if available.

Experimental setup:

BL14.1:
- Photon flux: 1.4x10¹¹ Phot/sx100mAx0.05%BW at sample position
 (0.1-1 sec exposure time per frame)
- User defined beam shaping from 50µm-100µm diameter possible
- Pilatus 6M detector, 141mm-680mm max. distance from the sample
- Microdiffractometer (MD2) with Mini-kappa goniometer
- Automatic sample changer (CATS), 90 sample storage capacity
(SPINE-Pin & EMBL sample magazine compatibility)
- 32-core XEON-CPU server, with 10Gb uplink to Pilatus 6M
- Data collection control via MXCuBE
- EDNA
- Common MX-software installed including XDS, iMOSFLM, CCP4,
 Phenix, SHELXC-D-E, etc.
- Automated data processing with XDSAPP
- Remotely controlled cryo-shutter for crystal annealing
- Pressure chamber for noble gas derivatization (Xe, Kr
 available upon request)
- AMPTEK-XRF detector and XFEPLOT software available

We are also offering the hard- and software environment for
carrying out:
- UV-RIP experiments at BL14.1. For further information, please visit:

https://www.helmholtz-berlin.de/forschung/oe/np/gmx/ancillary-facilities/uvrip_en.html

BL14.2:
- Photon flux: 1.9x10¹¹ Phot/sx100mAx0.05%BW at sample position
 (0.1-1 sec exposure time per frame)
- Pilatus 3S-2M detector with 1000 micron Si sensor thickness,
 55mm-600mm distance from the sample
- Nanodiffractometer (DESY P11 design) and on-axis sample microscope
- User defined beam shaping from 30µm-150µm diameter possible
- GROB sample changer for SPINE and UNIPUCK support
- 60-core XEON-CPU server, with fibre channel SAN up-link data
 processing environment
- Common MX-software installed including XDS, iMOSFLM, CCP4, Phenix,
 SHELXC-D-E, etc.
- Automated data processing with XDSAPP
- Amptek XRF detector
- Pressure chamber for noble gas derivatization (Xe, Kr available
 upon request)
- Ultra high performance stereo microscope Leica M205A, 20-255x zoom,
 8 Mpixel CCD-camera
- UV-Microsprectrophotometer offline setup available
- AMPTEK-XRF detector and XFEPLOT software available

BL14.3:
- Photon flux: 4x10exp10 Phot/sx100mAx0.05%BW at sample position
 (3-20 sec exposure time per frame)
- Rayonix MX-225 X-ray detector, 54mm-450mm distance from the
 sample, 30 deg 2-Theta possible
- MD2S microdiffractometer
- REX nozzle changer
- 60-core XEON-CPU server, with fibre channel SAN up-link data
 processing environment
- EDNA installed and available
- Common MX software installed including XDS, iMOSFLM, CCP4, Phenix,
 SHELXC-D-E, etc.
- Automated data processing using XDSAPP
- Remotely controlled cryo-shutter for crystal annealing
- HC1lab dehydration device installed (please specify HC1-beamtime
 in your proposal if needed)
- Pressure chamber for noble gas derivatization (Xe, Kr available
 upon request)
- Ultra high performance stereo microscope Leica M205A, 20-255x zoom,
 8 Mpixel CCD-camera

S1-biolab facilities (separate registration required):
- Protein production and purification
- Nanoliter 96 well crystallization plate formulation and storage at
 5 °C and 20 °C
- Biophysical characterization with real time PCR (thermofluor assay)

The HZB-MX group is also providing expert assistance as well as
access to a library of fragments for carrying out crystallographic
fragment-screening experiments. For more information please contact
mswe...@helmholtz-berlin.de.

Please visit our web page 
www.helmholtz-berlin.de/bessy-mx to 
obtain
updated information about our experimental setup and other
requirements.

Manfred Weiss and the HZB-MX team

--
Dr. Manfred S. Weiss
Macromolecular Crystallography
Helmholtz-Zentrum Berlin
Albert-Einstein-Str. 15
D-12489 Berlin
Germany



Helmholtz-Zentrum Berlin für Materialien und Energie GmbH

Mitglied der Hermann 

[ccp4bb] Vacancy at Diamond: Senior Support Scientist (I04-1, XChem)

[Frank von Delft]

Hello all

I'm recruiting a Senior Support Scientist to my beamline I04-1 at 
Diamond.  Apart from MX user support (obviously), the position will help 
work on automating more complicated kinds of data collection and 
figuring out how to get more data faster, especially for XChem fragment 
screening but also beyond.


Technically-minded crystallographers that enjoy teaching or training 
people, but also like hands-on wires-and-cables style work and/or 
mucking around with programming or scripting, will enjoy this job. 
Diamond is incredibly collegial, with a terrific user community.


The ad is here:
https://vacancies.diamond.ac.uk/vacancy/senior-support-scientist-356961.html

Frank


--
Prof Frank von Delft

Principal Beamline Scientist: I04-1
Diamond Light Source
+44 1235 778997 (office: M,T,T)
+44 7471 026103 (mobile)

Associate Professor Principal Investigator: Protein Crystallography
Structural Genomics Consortium
Oxford University
+44 1865 617583 (office: W,F)



To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1