Re: [ccp4bb] Off topic question

2019-01-03 Thread Georg Hochberg
Dear Reza, CD hit will do exactly that. Cheers, Georg Sent from my iPhone On Jan 3, 2019, at 2:41 PM, Reza Khayat mailto:rkha...@ccny.cuny.edu>> wrote: ​Hi, Happy new year to all! A bit of an off topic question. Does anyone know of a method/program to extract the most distinct "n"

Re: [ccp4bb] Off topic question

2019-01-03 Thread Javier Gonzalez
Hi Reza, happy new year! The choice would depend on your alignment (aminoacid or nucleotides? are the sequences closely or distantly related? is it a large alignment? are there many gaps?)... Anyway, I think the safest, unbiased way to determine a group of outliers might be to compute a

Re: [ccp4bb] Off topic question

2019-01-03 Thread Ethan A Merritt
On Thursday, January 3, 2019 12:40:05 PM PST Reza Khayat wrote: > ?Hi, > > > Happy new year to all! A bit of an off topic question. Does anyone know of > a method/program to extract the most distinct "n" (n>2) sequences from a > sequence alignment? Thanks. If these putative "most distinct"

Re: [ccp4bb] Off topic question

2019-01-03 Thread Zhijie Li
PCA? On Jan 3, 2019, at 3:41 PM, Reza Khayat mailto:rkha...@ccny.cuny.edu>> wrote: ​Hi, Happy new year to all! A bit of an off topic question. Does anyone know of a method/program to extract the most distinct "n" (n>2) sequences from a sequence alignment? Thanks. Best wishes, Reza

[ccp4bb] Off topic question

2019-01-03 Thread Reza Khayat
?Hi, Happy new year to all! A bit of an off topic question. Does anyone know of a method/program to extract the most distinct "n" (n>2) sequences from a sequence alignment? Thanks. Best wishes, Reza Reza Khayat, PhD Assistant Professor City College of New York Department of Chemistry

[ccp4bb] CCP-EM Spring Symposium: 29 April - 1 May, Nottingham University

2019-01-03 Thread Colin Palmer - UKRI STFC
Dear all, We are pleased to announce that registration is now open for the fifth annual CCP-EM Spring Symposium. Sign up on the event website before 25 January for an Early Bird discount: http://www.cvent.com/d/xbqsqk The Spring Symposium aims to provide a forum to highlight state of the art

Re: [ccp4bb] opentrons pipetting robot

2019-01-03 Thread Paul Paukstelis
I have long thought about developing an open-source crystallization robot based on the now ubiquitous 3D printing linear motion systems. They are certainly plenty precise for doing crystallization plating. Probably having two heads, a 12-channel syringe system for screen dispensing and a second

Re: [ccp4bb] Refinement with native and anomalous data

2019-01-03 Thread Eleanor Dodson
I think any decision depends on the resolution of your two data sets. If they are very different I would choose the higher resolution one. If that is the Anom data then I would use the anom signal at least in the first cycles to improve the phases.. Eleanor On Thu, 3 Jan 2019 at 14:59, Piotr

[ccp4bb] Job opening

2019-01-03 Thread Ronnie
A career opportunity if you want to pivot your structure Biology experience for something new.Check out this job: Protein Antibody Engineer https://www.linkedin.com/jobs/view/1031138315 To unsubscribe from the CCP4BB list,

Re: [ccp4bb] opentrons pipetting robot

2019-01-03 Thread Tim Craig
The opentron liquid handler is currently in what I would consider an alpha stage of development in both hardware and software, when compared to more expensive liquid handlers produced by companies like tecan, hamilton, agilent, etc. It is considerably less useful due to the lack of software

[ccp4bb] Refinement with native and anomalous data

2019-01-03 Thread Piotr Wilk
Dear CCP4 experts, I'd like to ask your opinion about using anomalous signal in refinement of crystal structures in addition to using high resolution native data. I am working on a series of structures for which I have collected two data sets (from the same crystal): 1 - native with higher

[ccp4bb] 12th CCP4/APS Crystallographic School in the US

2019-01-03 Thread Xu, Qingping
Dear Colleagues, We are pleased to announce the 12th annual CCP4 crystallographic school “From data collection to structure refinement and beyond” will be held on June 17-24, 2019 at Advanced Photon Source (APS), Argonne National Laboratory (ANL), near Chicago, Illinois, USA. All details can

Re: [ccp4bb] 2019 New Year’s resolutions of a cryo-EM newbie

2019-01-03 Thread Carter, Charlie
I, for one, was very happy to see Marin’s remark, which pointed something everyone is shoving under the rug. By far the most significant source for information to US citizens is FOX news, which is pay rolled to brainwash as many as they reach. Even public television and NPR have been hammered