Re: [ccp4bb] [6HR5] collected on an Eiger so Rmerge not relevant

2019-07-31 Thread Kay Diederichs
First of all, you are of course correct, Rmerge (as Rmeas, Rpim, CC1/2, I/sigma ...) is not detector-dependent. Second, when looking at the "experiment" section of the PDB deposition, I note that some Rmerge values are even given there! The statistics there are dubious, e.g. seemingly the

Re: [ccp4bb] [EXTERNAL] [ccp4bb] [6HR5] collected on an Eiger so Rmerge not relevant

2019-07-31 Thread Ivan Shabalin
Hi Clemens, I fully agree with you, especially on overall data collection strategy and on image deposition. It looks like an interesting and mysterious case. Mean I Over Sigma is reported as 2.2 - I'm curious why resolution was not extend. Since they disregarded Rmerge (a reasonable thing

Re: [ccp4bb] [EXTERNAL] [ccp4bb] [6HR5] collected on an Eiger so Rmerge not relevant

2019-07-31 Thread Clemens Vonrhein
Hi, On Wed, Jul 31, 2019 at 07:11:10PM +0100, Weston Lane wrote: > Thanks for the response. I did look at the multiplicity of the > datasets in their table and while I suppose 6.9x redundancy is sort > of high for P2 spacegroup it's actually lower than some of the other > datasets (presumably

[ccp4bb] RESEARCH SCIENTIST POSITION in INSTITUTE FOR PROTEIN DESIGN

2019-07-31 Thread StrBio
RESEARCH SCIENTIST ENGINEER 1 Req #: 170450 Department: BIOCHEMISTRY - INSTITUTE FOR PROTEIN DESIGN Posting Date: 07/31/2019 Closing Info: Open Until Filled Salary: Salary is commensurate with education and experience Shift: First Shift Notes: As a UW employee, you will enjoy generous benefits and

[ccp4bb] Postdoctoral Position Available at Rutgers University

2019-07-31 Thread Matthew Neiditch
An NIH-funded postdoctoral position is available at Rutgers University - New Jersey Medical School to study the structural basis of bacterial competence, more specifically the movement of DNA across bacterial membranes during natural transformation. In addition to a recent Ph.D., the ideal

Re: [ccp4bb] [EXTERNAL] [ccp4bb] [6HR5] collected on an Eiger so Rmerge not relevant

2019-07-31 Thread Weston Lane
Edward, Thanks for the response. I did look at the multiplicity of the datasets in their table and while I suppose 6.9x redundancy is sort of high for P2 spacegroup it's actually lower than some of the other datasets (presumably non-Eiger) in the table with good overall Rmerge (e.g. a C2

Re: [ccp4bb] [EXTERNAL] [ccp4bb] [6HR5] collected on an Eiger so Rmerge not relevant

2019-07-31 Thread Edward A. Berry
I think it is not really the detector, but the strategy. If you decrease exposure time by a factor of ten and make up for it by 10 x higher redundancy, then obviously R-merge, which is a measure of accuracy of the individual frames, is going to suffer. Chi^2 statistics can distinguish this

[ccp4bb] [6HR5] collected on an Eiger so Rmerge not relevant

2019-07-31 Thread Weston Lane
I was looking at the following structure in the PDB: http://www.rcsb.org/structure/6HR5 I noticed that the R/Rfree stats were pretty high for 2.9A resolution so I followed up by looking for the "Table 1" statistics in the journal article. Link to article:

[ccp4bb] Conference in Rome on CryoElectron Microscopy in Structural Biology, 10-11 October 2019

2019-07-31 Thread Adriana E. Miele
Dear CCP4BBoarders, on behalf of the Organizing committee we would like to invite you to the Conference “CryoElectron Microscopy in Structural Biology: paving the way towards biomedicine and biotechnology”, jointly organised by Sapienza University of Rome and CNR Institute of Molecular Biology