Hi Lindsey,
As I mentioned to you in the separate email, 180 degrees for each half is
too little.
Here I'll try to explain some more about SAD vs. MAD:
What I have observed at our beamlines is that the majority of those who
collect MAD data, do it as as an afterthought of SAD. Priority in these
Hi Lindsey,
My couple cents:
1) Make sure to use "Auto-corrections" option for scaling with HKL. This
option is great for extracting the anomalous signal.
2) Im also with Andreas and Nukri on collecting 360 at lower dose and
leaving the inverse beam for later.
Best,
Ivan
With best
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Dear Natesh,
as Petri pointed out this is a common behavior for proteins/protein
complexes that deviate substantially from a "globular" shape. It is very
typical for elongated proteins/protein complexes containing e.g. extended
coiled-coil regions. These samples will elute much earlier from a SEC
Because MALS can capture distinct migrant form of the same protein,
sometimes protein with disorder and elongated structure behave differently
in SEC. In SEC we can't distinguish them. Whereas MALS have scattering at
three different angles, by that we can captures those multiple forms of
the
Dear Lindsey,
I'm all with Nukri on this one. 12 Se atoms (whose incorporation you've
shown) should be plenty to give you enough anomalous signal to phase the
data and solve the structure. That's why I would do the simplest
experiment first. Collect 360° of data at the peak energy, maybe a bit
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Hi,
that's typical behaviour for an elongated/disordered molecule, given that SEC
separates based on hydrodynamic radius, not MW.
Petri
Petri Kursula
--
Professor
--
Department of Biomedicine
University of Bergen, Norway
http://www.uib.no/en/rg/petrikursula
What do you mean by mass from SEC? SEC is not a mass determination method
Sent from my iPhone
On 27 Aug 2019, at 06:57, Natesh Ramanathan
mailto:nat...@iisertvm.ac.in>> wrote:
Dear Friends,
Can you share your experience with examples of MALS giving lower
molecular weight (Eg.
Natesh,
Did you use an experimentally determined dn/dc value?
Is it a glycoprotein, or does it have some other modification?
Default dn/dc values are not appropriate for glycoproteins, and will give rise
to incorrect Mwt values.
Dave
--
Dr David C. Briggs
Senior Laboratory Research Scientist
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