Re: [ccp4bb] Lattice-translocation defect (LTD)

2020-02-11 Thread Wim Burmeister
Hello, do you have some details about the space group ? Did the integration not miss any sports ? I would rather think of an ncs close to crystallographic symmetry, or maybe some twinning problem. I guess these are Pilatus data, can you combine the frames

[ccp4bb] problem in structure solution of multidomain protein

2020-02-11 Thread Rajnesh Kumari Yadav
Hello everyone, I am working on a protein which have 5 domain in it, we have its 4 domain structure and 3.0 Angstrom data of 5 domain protein crystal. While doing the Molecular replacement we got solution with four domain with good electron density and space for last domain without any

[ccp4bb] problems loading images in iMosflm under CCP4

2020-02-11 Thread Annette Herta Erbse
Hi Everyone, I apologize in advance I am probably doing something stupidly wrong, but I have problems adding images taken with a Dectris Pilatus 200K detector into iMosflm. The files are .img files. I get the message "Error reading image header. Message from Mosflm is Incorrect size of image

Re: [ccp4bb] Lattice-translocation defect (LTD)

2020-02-11 Thread 00000c2488af9525-dmarc-request
I was wondering why you believe it is a lattice defect rather than NCS. How well does the structure refine and does it have NCS? Do the twinning tests suggest anything?Jon CooperOn 11 Feb 2020 21:31, Daniele de Sanctis wrote:Hi all,I'm currently dealing with what I think it is a case of LTD

[ccp4bb] Lattice-translocation defect (LTD)

2020-02-11 Thread Daniele de Sanctis
Hi all, I'm currently dealing with what I think it is a case of LTD (off-origin Patterson peak, with vector along w of ~ 7A and electron density map showing a "ghost" map shifted by 7 A). I saw there are quite a few cases reported in literature (for example Hare et al, 2006), but what I could

[ccp4bb] Post doc position available at Florida State University

2020-02-11 Thread Adarsh Kumar
Dear all One postdoctoral position is available immediately in an enzymology laboratory to elucidate mechanisms of DNA/RNA synthesis, DNA lesion bypass, and base excision repair catalyzed by various human and viral DNA/RNA polymerases, and to mechanistically investigate gene editing enzymes

[ccp4bb] Cryo-FIB/SEM & Cryo-ET postdoctoral positions in New York, $60 - 80K per year

2020-02-11 Thread Anthony Fitzpatrick
The Fitzpatrick lab at Columbia's Zuckerman Institute has multiple openings for postdoctoral research associates to lead projects combining serial-section electron microscopy, correlative light/electron microscopy, advanced cryo-focused ion beam specimen preparations, cryo-electron tomography and