Re: [ccp4bb] I cannot download xds

Hello, it downloaded to my phone from the link on this page:http://xds.mpimf-heidelberg.mpg.de/html_doc/downloading.htmlIt looks alright: http://u.cubeupload.com/jbcooper/20200628034613.jpgJon CooperOn 28 Jun 2020 01:39, Murpholino Peligro wrote:```~/Downloads$ aria2c

[ccp4bb] I cannot download xds

``` ~/Downloads$ aria2c ftp://ftp.mpimf-heidelberg.mpg.de/pub/kabsch/XDS-INTEL64_Linux_x86_64.tar.gz 06/27 19:36:10 [NOTICE] Downloading 1 item(s) [#3065b2 0B/10MiB(0%) CN:1 DL:0B] ``` It stays there forever. Is there an alternative download url? Thanks

Re: [ccp4bb] Looking for suggestions with protein expression

Dear Umar, Have you determined the Fe-S cluster content of the as-isolated protein? If you use common BL21 cells for protein production, the protein is most likely not fully maturated (the majority of the protein does probably not contain a Fe-S cluster). Thus, you may have free Cys (if the

Re: [ccp4bb] Looking for suggestions with protein expression

Hi Umar Artem is right, we should have some more infos about how you proceed after disrupting the cells. The part I find curious is your usage of "usually". Usually soluble when? Before you centrifugate the sample? Before you go into the äkta? That would be a first hint to know what you mean

Re: [ccp4bb] Looking for suggestions with protein expression

Glutathione beads for GST tagged protein. Umar On Sat, 27 Jun 2020, 6:56 pm John Newitt, wrote: > On Jun 27, 2020, at 3:15 AM, Umar Farook wrote: > > > >  > > Dear All, > > > > Sorry for an offtopic question, your suggestions are highly appreciated. > > > > We have been working on iron

Re: [ccp4bb] Looking for suggestions with protein expression

On Jun 27, 2020, at 3:15 AM, Umar Farook wrote: > >  > Dear All, > > Sorry for an offtopic question, your suggestions are highly appreciated. > > We have been working on iron sulfur cluster binding protein, which is usually > expressed as a nice soluble protein expressed in BL21 cells but

Re: [ccp4bb] Looking for suggestions with protein expression

Hi It is hard to give specific advice without knowing details about your protein. There are multiple reasons that may be responsible for it's behavior (fundamentally the simplest reason is that proteins by and large are not designed by Nature to be purified and handled) so the more you share with

Re: [ccp4bb] How many microfocus beamlines are in the world?

Hi, At the ISDSB 2019 in Osaka last November I asked Stephen Burley about the BioSync pages, commending how useful they are. Stephen said they had asked for a budget line in their last renewal to help keep the pages up to date but it had been cut. At the next renewal, or earlier, we should

Re: [ccp4bb] How many microfocus beamlines are in the world?

Thanks for the clarification Dave, I completely agree that this is a very useful resource, but I can also understand why things are not always kept fully up-to-date. Perhaps it would be useful if people notify the appropriate beam line team when they notice that the details on the Biosync

Re: [ccp4bb] How many microfocus beamlines are in the world?

Dear Andrew, Rasmus, The pages are updated by the beamline teams typically. This is another set of pages to keep up to date alongside their own websites (and others) so sometimes can get overlooked when updates are made to beamlines or staff change. However in spite of some out of date

Re: [ccp4bb] How many microfocus beamlines are in the world?

Dear Rasmus, Yes, I remember that the last time I looked at it (which is probably 2 years ago) I also found beamlines where the details were clearly not up-to-date. I see from the website that BioSync was funded by NIH and NIGMS from 2016-2018, so maybe there is no funding

Re: [ccp4bb] How many microfocus beamlines are in the world?

Dear All, The link http://biosync.rcsb.org/index.jsp looked very interesting, but a cursory look found the following line in a beamline description: "Next proposal submission period Mid 2013" Not 100% up to date, then. Yours, Rasmus On 25/06/2020 09:36, Andrew Leslie wrote: Dear

[ccp4bb] Looking for suggestions with protein expression

Dear All, Sorry for an offtopic question, your suggestions are highly appreciated. We have been working on iron sulfur cluster binding protein, which is usually expressed as a nice soluble protein expressed in BL21 cells but aggregated in the affinity column itself and unable to recover from it.