After taking images at room temp and assuming good diffraction then, I would
kick up the PEG400 concentration and as a first go on the PEG400 condition and
try low molecular weight PEG's as a cryo protectant for the other two
conditions.
Good Luck, been there and have exposed many crystals in
Dear Everton and Nukri,
Thank you for your kind reply, we were also speculating that there could be
some issues with cryoprotection.
We have used two cryoprotectants naming; Parabar (10312) and Paraffin Oil
from Hampton Research company. We have also used glycerol and respective
mother liquors
Dear Saurab,
It's a shame these crystals do not diffract well - they look so good!
I assume the diffraction you saw was recorded at cryo temperatures? If so,
it is possible that you may be damaging the crystals during cryo-pretection
and cryo-cooling. Before you go too deep into changing the
Dear Saurabh,
Your crystals are gorgeous!
Which cryoprotectant did you use?
Best,
Everton.
Sent from my iPhone
> On Mar 21, 2021, at 11:04 AM, Saurabh Upadhyay
> wrote:
>
>
> Dear All,
>
> I am trying to crystalize a protein, which occurs in monomeric (60kDa) and
>
