[ccp4bb] Register for the 2021 ACA Fundamentals of Single-Particle Cryo-EM Workshop

Please join us at our comprehensive single-particle cryo-EM workshop – Fundamentals of Single-Particle Cryo-EM – at the virtual American Crystallographic Association 71st annual meeting "Structural Science Awakens". This cryo-EM focused workshop will be held virtualy on 2 days: 8/17-18 from

[ccp4bb] Postdoctoral positions at UT Southwestern, Dallas

On behalf of Prof. Nicastro: The Nicastro lab

Re: [ccp4bb] pictures in emails

Dear All, what about linking to any free cloud service for large attachments? Isn't CCP4 7.1 already such a cloud? Best, marco Marco Patrone – PhD Biocrystallography Unit Division of Immunology, Transplantation, and Infectious Disease (DITID) DIBIT – San Raffaele Scientific Institute Via

Re: [ccp4bb] pictures in emails

Hi Frank, CCP4bb-ers, While the progress in the digital world in 2021 over the 90s is undeniable (which, unfortunately cannot be said about the analog world), we should keep in mind that some institutional accounts may filter out the "suspiciously" large emails. I would not be surprised if some of

Re: [ccp4bb] pictures in emails

Ahem.  Ed, Tim - the 90s called, they want want their disk space and email clients back. As a co-perpertrator of vast volumes of detector data, and religious proponent of text /formatting/ as inseparable part part the message, I disagree that in the year 2021, your suggestion still conforms

Re: [ccp4bb] Negative electron density

Just for completeness: BUSTER includes an automatic so-called "void correction" that tries to detect those incorrectly bulk-solvent filled cavities and correct for it. Nothing for you to do extra, since that correction is on by default ... and works as expected in a lot of cases as far as we can

Re: [ccp4bb] 60 kDa contamination in Rosetta cells

Hvvxbxhxxhhfjxjdjdhhdydfyhat the 60 kDa is not your biggest concern. Optimize soluble protein expression and pay attention to cell lysis. Is the sample remaining cold throughout the process? Are you using enough volume of buffer relative to solid cell pellet? > > Also, from the gel it does not

Re: [ccp4bb] pictures in emails

Hi Ed, dear ccp4bbs I fully support your email! Most email clients have a tick box to include a plain text version in addition of the html-version in their preferences. Reading plain text emails instead of the html-version (which is also an available option for most email clients) is a very

[ccp4bb] Postdoc positions Leicester, UK

Dear all, We are looking for postdoctoral researchers to join our lab our lab at the Leicester Institute for Structural and Chemical Biology, UK. The positions are funded by a 5 year Wellcome Trust Investigator award to work on the regulation of gene expression and 3D genome organisation. For

[ccp4bb] Application deadline approaching - Virtual CCP4 Protein Crystallography Summer School 2021 (6th-10th September)

Dear colleagues, We are pleased to announce the Virtual CCP4 Protein Crystallography Summer School 2021 to be hosted by the John Innes Centre (Norwich, UK). The school will take place from Monday 6 September – Friday 10 September inclusive and is intended for students and researchers new to