[ccp4bb] Faculty Positions at Montana State University

[Blake Wiedenheft]

Dear CCP4 Community,



Montana State University (MSU) invites applications for 3 tenure-track
faculty positions in neuroimmunology (see advertisement below).  We are
particularly interested in candidates that intend to develop research
programs that include structural biology. Relevant core facilities at MSU
include X-ray crystallography, NMR, mass spectrometry, and a new Talos
Arctica microscope equipped with a Gatan K3 camera.  In addition to this
cluster hire, MSU will also be announcing a cryo-EM specific position soon.
Please help us spread the word.





*Three Faculty Positions in the Department of Microbiology & Cell Biology
at Montana State University*

This is where research and discovery happen. Located in the heart of the
Rocky Mountains, Bozeman, Montana offers a healthy lifestyle and a world
class research university that facilitates innovation and scholarship. The
Department of Microbiology & Cell Biology at Montana State University (MSU)
invites applications for three tenure track faculty positions in the field
of Neuroimmunology. This cluster hire aims to attract a diverse cohort of
exceptional individuals that will establish nationally recognized, and
externally funded research programs that use innovative new methods to
interrogate dynamic interactions at the interface of Neurology and
Immunology. Areas of interest include, but are not limited to, fundamental
and applied biology, immunology, neurology, pathology, psychiatry,
parasitology, aging, structural biology, and virology. Successful
applicants will integrate their research programs with undergraduate and
graduate student instruction and participate in professional service
activities. We are particularly interested in individuals whose work
complements and strengthens the research interests of MSU faculty,
including those focused on neurology, developmental biology, immunology,
microbial physiology and ecology, molecular evolution, virology, biomedical
microbiology, environmental health, structural biology, and host-pathogen
interactions. The Department of Microbiology & Cell Biology offers a
dynamic research and teaching environment with state-of-the-art facilities
for infectious disease (e.g., BSL3), flow cytometry, light and
cryo-electron microscopy, histology, and small and large animal research.
The University supports comprehensive cores in metabolomics, proteomics,
imaging, structural biology, and genomics/bioinformatics. This position is
supported by a competitive institutional salary, a generous start-up
package, and state-of-the-art research facilities. The job description and
application materials are available online (
https://jobs.montana.edu/postings/25947).



Screening of applications will begin on October 15th, 2021 and screening
will continue until an adequate applicant pool has been established. Montana
State University values diverse perspectives and is committed to
continually supporting, promoting, and building an inclusive and culturally
diverse campus environment. MSU recognizes the importance of work-life
integration and strives to be responsive to the needs of dual career
couples. Equal Opportunity Employer, Veterans/Disabled



Please contact Kelley Kane (kelleyk...@montana.edu*) *for more information.

-- 
Blake Wiedenheft, PhD
Associate Professor
Dept. Microbiology and Cell Biology
Montana State University
Bozeman MT 59717
tel: 406 994 5009
fax: 406 994 4303
Wiedenheft Lab Website 
Google.Scholar.Wiedenheft




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[ccp4bb] last day to register for the SSRL/LCLS User Meeting

[Mooers, Blaine H.M. (HSC)]

Dear CCP4BB,

Today is the last day to register for the SSRL/LCLS User Meeting that starts on 
Monday.
It is free.  https://events.bizzabo.com/SLAC-UsersMeeting-2021/home.

The poster session runs 12 - 1 PM Pacific Time M-Th.
You use the gathertown software to attend it.
Navigation of the poster session is like playing a 1990s computer game.

I was on the poster judging team. 
We spent this week judging the posters virtually.
We made our selections of the finalist today.
All of the semifinalist posters were excellent.

Best regards,

Blaine
SSRL UEC member and past chair.

Blaine Mooers, Ph.D.
Associate Professor
Department of Biochemistry and Molecular Biology, College of Medicine
Director of the Laboratory of Biomolecular Structure and Function
Academic Director, Biomolecular Structure Core, COBRE in Structural Biology
Member, Cancer Biology Program, Stephenson Cancer Center
University of Oklahoma Health Sciences Center

Mailing Address:
975 NE 10th Street, BRC 466
Oklahoma City, OK 73104-5419
Office: 405-271-8300 Lab: 405-271-8312 

Websites:
Faculty page: 
https://basicsciences.ouhsc.edu/bmb/Faculty/bio_details/mooers-blaine-hm-phd
BSC-OKC (LBSF): 
https://research.ouhsc.edu/Core-Facilities/Laboratory-of-Biomolecular-Structure-and-Function
COBRE in Structural Biology: https://www.ou.edu/structuralbiology


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Re: [ccp4bb] PDB file secondary structure annotations

[Nigel Moriarty]

Neno

Can you tell me what version of Phenix you are using? and send me the file
in question? Off-list.\

NB. Any files sent to me will be held in strictest confidence.
Cheers

Nigel

---
Nigel W. Moriarty
Building 33R0349, Molecular Biophysics and Integrated Bioimaging
Lawrence Berkeley National Laboratory
Berkeley, CA 94720-8235
Phone : 510-486-5709 Email : nwmoria...@lbl.gov
Fax   : 510-486-5909  Web  : CCI.LBL.gov
ORCID : orcid.org/-0001-8857-9464


On Fri, Sep 17, 2021 at 4:36 AM Robbie Joosten 
wrote:

> Yes, you can use DSSP 4.0 (https://github.com/PDB-REDO/dssp) to directly
> annotate your structure model in PDB or mmCIF format. The latter is
> recommended.
>
> Cheers,
> Robbie
>
> > -Original Message-
> > From: CCP4 bulletin board  On Behalf Of Neno
> > Vuksanovic
> > Sent: Thursday, September 16, 2021 22:36
> > To: CCP4BB@JISCMAIL.AC.UK
> > Subject: [ccp4bb] PDB file secondary structure annotations
> >
> > Dear All,
> >
> > I noticed that files available in the Protein Data Bank contain secondary
> > structure information which allows for them to be imported in a program
> such
> > as BioRender in order to make structural figures. However, the PDB files
> > generated by PHENIX for structures that I haven't deposited yet do not
> contain
> > such information so BioRender displays them as coils. Is there an easy
> way to
> > add secondary structure information to a PDB file?
> >
> > Best Regards,
> > Neno
> >
> >
> > 
> >
> >
> > To unsubscribe from the CCP4BB list, click the following link:
> > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1
>
>
> 
>
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>
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[ccp4bb] Vacancy: Group Leader role in Science IT

[Ashton Alun (PSI)]

We have a new opportunity for someone to join the Science IT Infrastructure and 
Services department to contribute and lead a group tasked with the software 
environment for the operations and rapidly evolving developments needed for 
experiments at the Swiss Light Source SLS and Switzerland's X-ray free-electron 
laser SwissFEL.

Further details:
Role:  Group Leader: Experiment IT Development and Operations
Closing Date: 10/10/21
https://www.psi.ch/en/pa/job-opportunities/47472-group-leader-experiment-it-development-and-operations

Please don't hesitate to contact me if you have any questions.

Other and similar roles at PSI available at: 
https://www.psi.ch/en/pa/job-opportunities
__
Dr Alun Ashton
Head of Science IT Infrastructure and Services (AWI) Department
Paul Scherrer Institut
WHGA/036
Forschungsstrasse 111
5232 Villigen PSI
Schweiz
Telefon: +41 56 310 44 81





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Re: [ccp4bb] PDB file secondary structure annotations

[Robbie Joosten]

Yes, you can use DSSP 4.0 (https://github.com/PDB-REDO/dssp) to directly 
annotate your structure model in PDB or mmCIF format. The latter is 
recommended. 

Cheers,
Robbie

> -Original Message-
> From: CCP4 bulletin board  On Behalf Of Neno
> Vuksanovic
> Sent: Thursday, September 16, 2021 22:36
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] PDB file secondary structure annotations
> 
> Dear All,
> 
> I noticed that files available in the Protein Data Bank contain secondary
> structure information which allows for them to be imported in a program such
> as BioRender in order to make structural figures. However, the PDB files
> generated by PHENIX for structures that I haven't deposited yet do not contain
> such information so BioRender displays them as coils. Is there an easy way to
> add secondary structure information to a PDB file?
> 
> Best Regards,
> Neno
> 
> 
> 
> 
> 
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1




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[ccp4bb] extended deadline: Postdoc vacancy Gros-group Utrechtn university

[Harma Brondijk]

Hi All

 

The deadline to apply for the position of Postdoctoral Researcher for
cryo-EM studies of large protein complexes involved in cellular immunity in
the group of Piet Gros at Utrecht University, The Netherlands. Has been
extended to October 3rd. See the attached PDF for details of the position
and a link to apply.

 

Please feel free to forward this message to anyone who might be interested
in this position.

 

Best,

Harma Brondijk




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Postdoc vacancy-Gros group.pdf
Description: Adobe PDF document

[ccp4bb] Coot 0.9.6.EL Linux Ubuntu

[Andrea Smith]

Hi,

I updated my ccp4 and it came with Coot 0.9.6.EL where the sequence view does 
not show the numbers of amino acids in the sequence and some letters overlap - 
anyone having the same problem? Do I need to install some other version?

Thanks,
Andrea



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Re: [ccp4bb] AW: [ccp4bb] Phosphatase enzymatic assay

[Andrea Moretti]

Dear all,

Thanks a lot for all your nice suggestions, I really appreciate them.

I am now digging through all the methods you suggest trying to find the one 
that will solve my enzimatic issue.
For the ones that asked what was not working, I am trying to replicate some 
data but with not much success until now.

Thanks again for your help and have a nice day.

Andrea

> Il giorno 16 set 2021, alle ore 17:11, Schreuder, Herman /DE 
>  ha scritto:
> 
> 
> It may also be possible to separate the different phosphorylated forms on an 
> isoelectric focusing gel.
> Best,
> Herman
>  
> Von: CCP4 bulletin board  Im Auftrag von Patrick Loll
> Gesendet: Mittwoch, 15. September 2021 15:12
> An: CCP4BB@JISCMAIL.AC.UK
> Betreff: Re: [ccp4bb] Phosphatase enzymatic assay
>  
> Phos-tag gels are an option (albeit a fiddly one).
> Cheers,
> Pat Loll
>  
>  
> ___
>  
> Patrick J.  Loll, PhD (he, him, his)
> Professor of Biochemistry & Molecular Biology
> Drexel University College of Medicine
> Room 10-102 New College Building
> 245 N. 15th St.
> Philadelphia, PA 19102-1192 USA
>  
> (267) 359-2653
> pj...@drexel.edu
>  
> 
> 
> On 15 Sep 2021, at 8:28 AM, Andrea Moretti  wrote:
>  
> Dear CCP4 community,
> 
> sorry for the off topic question but I am struggling with enzymatic assays 
> and I would need your help.
> 
> I am trying to measure dephosphorylation of a phosphorylated kinase by a 
> ser/thr phosphatase. So far I tried to measure it by malachite green assay, 
> anti pSer/pThr antibody and PNPase coupled assay but nothing really worked 
> for me. NMR is unfortunately not an option since I can't make enough of the 
> proteins.
> 
> So any hints on phosphatase assays would be helpful.
> Thanks!
> 
> Best wishes,
> Andrea
> 
> 
> -- 
> Andrea Moretti
> Structural Plant Biology Laboratory
> Department of Botany and Plant Biology
> Sciences III
> University of Geneva
> 30 Quai E. Ansermet
> 1211 Geneva
> Switzerland
> Email: andrea.more...@unige.ch
> Phone: +41 22 379 3029 (office)
> web:http://structplantbio.org
> 
> 
> 
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>  
> Patrick Loll
> pjl...@gmail.com
>  
>  
>  
>  
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> 
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[ccp4bb] PhD position in Vienna, Austria (University of Natural Resources and Life Sciences)

[Stefan Hofbauer]

Dear colleagues,

there is an opening for a PhD position in the Department of Chemistry, 
University of Natural Resources and Life Sciences, Vienna, Austria. 
The work will have a strong focus on structural biology methods, investigating 
a highly important enzyme involved in prokaryotic heme biosynthesis.
https://pubmed.ncbi.nlm.nih.gov/32440366/
https://pubmed.ncbi.nlm.nih.gov/34339636/

Please find information for this PhD position here:

https://euraxess.ec.europa.eu/jobs/682049
https://www.fwf.ac.at/de/service/fwf-jobboerse/stellenbeschreibung/jid/20210930-3501

Best regards,
Stefan

--
Stefan Hofbauer
Department of Chemistry
BOKU-University of Natural Resources and Life Sciences
Muthgasse 18
A-1190 Vienna, Austria
 
Phone:+43-1-47654-77258
e-mail: stefan.hofba...@boku.ac.at,
web:https://boku.ac.at/en/chemie/institut-fuer-biochemie-dchbc/group-obinger








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