Re: [ccp4bb] off-topic: pH meters

2021-09-20 Thread Roger Rowlett 
The meter doesn't matter so much as the electrode. For the meter, anything
with at least 3 pH multipoint calibration is sufficient for most purposes.
I usually bought something from our preferred university vendor a with very
large LCD displays for my aging eyes.

For protein work, and especially if using anything with Tris buffers, a
refillable, double junction Ag/AgCl reference combination electrode is a
must. Making stock 1M Tris buffer concentrate will wack out single junction
combination electrodes, sometimes permanently. I liked the Fisher
Accutuph/Accuphast electrodes because they were much more difficult for my
students to break, and they last for years. (My students never succeeded in
breaking one.) Gel-filled electrodes are garbage. Refillable double
junction electrodes are the way to go for stable readings, especially in
Tris, and minimizing heavy metal contamination.

Roger Rowlett
Gordon & Dorothy Kline Professoe, Emeritus
Department of Chemistry
Colgate University

On Mon, Sep 20, 2021, 4:57 PM Patrick Loll  wrote:

> Fellow protein biochemists:
>
> My ~30 year-old Beckman pH meter is finally showing its age, and I’m
> looking for a high-quality replacement that doesn’t cost insane amounts of
> money. My initial thoughts gravitate toward Mettler (it’s a name I trust,
> and some of their low-end instruments aren’t absurdly expensive); would
> anybody care to recommend other options?
>
> Much obliged for any suggestions (the more obsessive, the better).
>
> Cheers,
>
> Pat
>
>
>
> ---
> Patrick J. Loll, Ph. D.  (he, him, his)
> Professor of Biochemistry & Molecular Biology
> Drexel University College of Medicine
> Room 10-102 New College Building
> 245 N. 15th St., Mailstop 497
> Philadelphia, PA  19102  USA
>
> (215) 762-7706
> pjl...@gmail.com
> pj...@drexel.edu
>
> 
>
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[ccp4bb] off-topic: pH meters

2021-09-20 Thread Patrick Loll 
Fellow protein biochemists:

My ~30 year-old Beckman pH meter is finally showing its age, and I’m looking 
for a high-quality replacement that doesn’t cost insane amounts of money. My 
initial thoughts gravitate toward Mettler (it’s a name I trust, and some of 
their low-end instruments aren’t absurdly expensive); would anybody care to 
recommend other options?

Much obliged for any suggestions (the more obsessive, the better).

Cheers,

Pat


---
Patrick J. Loll, Ph. D.  (he, him, his)
Professor of Biochemistry & Molecular Biology
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA  19102  USA

(215) 762-7706
pjl...@gmail.com
pj...@drexel.edu



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[ccp4bb] CBMS Workbench - SAXS & MX - October 11 t0 13, 2021

2021-09-20 Thread Stojanoff, Vivian 
Dear All,

You are cordially invited to participate in the Center for Biomolecular 
Structure workbenches on solution scattering and macromolecular 
crystallography. Registration is open for the October 11 through 13, 2021 
session and is free.

https://www.bnl.gov/cbmsworkbench/

Choose between workbenches or select both and learn of alternative structure 
determination methods. Participants will have the opportunity to send samples 
to measure during the workbench. Data analysis will be shared. If you are 
planning to send samples, make sure to register early to obtain the necessary 
credentials for remote access to the beam lines.


Invited Speakers:

- Elspeth Garman (University of Oxford)
- Al Kikhney (Xenocs Nordic ApS.)
- Kay Diederichs (Universitaet Konstanz)
- Dorothee  Liebschner (Berkeley Lab)

To register follow the link:

https://www.bnl.gov/cbmsworkbench/

Registration is a two-step process if you are not a BNL guest. If you have a 
BNL guest appointment you need only to register for the workbenches.

Kind regards

Vivian

--
Vivian Stojanoff, PhD
Education, Training, Outreach
User Program
p 1(631) 344 8375
e nsls.lifescien...@gmail.com
w https://www.bnl.gov/ps/lifesciences/

Address:
Center for Biomolecular Structure
National Synchrotron Light Source II
Building 745
Brookhaven National Laboratory
Upton NY 11973




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[ccp4bb] Scientific Data Application Developer at RCSB PDB (Rutgers)

2021-09-20 Thread Jasmine Young 
RCSB PDB is looking for a highly motivated, scientific developer with 
domain experience in structural biology or bioinformatics or related 
fields. The candidate should be interested in structural biological data 
and enjoy engaging with other developers and scientists in a 
collaborative team environment.


The work at RCSB PDB focuses on data analysis, integration, and 
transformation, and the presentation and visualization of data using 
complex interactive graphical user interfaces. An important aspect is to 
provide users with the ability to search and explore the PDB data 
archive. Solutions are implemented using a wide range of components 
developed and maintained in-house, in addition to third-party tools, 
libraries, frameworks and technologies. The candidate should be 
comfortable working in a fast-changing environment, and able to 
demonstrate an ability to think creatively, generate new ideas, and 
implement solutions. The candidate should also show an eagerness and 
willingness to learn new skills and technologies.



   Requirements

The candidate should have experience working with Python, document 
databases (e.g. MongoDB), search and data access tools (e.g., 
ElasticSearch and GraphQL).


Scientific background in any of the following areas would be considered 
a major plus: Molecular Biology, Bioinformatics, Biochemistry, 
Chemistry, Genomics.


Experience working with large and complex scientific datasets would also 
be considered a plus.


While a scientific background is not an absolute requirement for the 
position, the candidate should indicate an interest in engaging with 
scientists and scientific content, and in working in an 
academic/research environment.



   Job Description

Responsibilities for this position will include:

 * Design, development, and deployment of modern data applications.
 * Analysis, refactoring and adaptation of legacy code to conform to
   new architecture designs.
 * Debugging, testing, troubleshooting.

Qualifications:

 * PhD or Masters degree in Computer Science, Biochemistry,
   Bioinformatics, or a related discipline, or a comparable combination
   of education and experience developing modern scientific data
   applications.
 * Excellent interpersonal, verbal, and written communication skills.


   Apply at RCSB PDB:

RCSB PDB is a friendly and collaborative working environment with 
excellent professional development opportunities. At Rutgers, this 
academic position offers New Jersey state benefits and a faculty-level 
salary.


Apply at https://jobs.rutgers.edu/postings/142529



--
Regards,

Jasmine

===
Jasmine Young, Ph.D.
Biocuration Team Lead
RCSB Protein Data Bank
Research Professor
Institute for Quantitative Biomedicine
Rutgers, The State University of New Jersey
174 Frelinghuysen Rd
Piscataway, NJ 08854-8087

Email: jasm...@rcsb.rutgers.edu
Phone: (848)445-0103 ext 4920
Fax: (732)445-4320
===




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[ccp4bb] Job posting: Full-Stack DevOps Developer position at EMBL Grenoble

2021-09-20 Thread Jose A. Marquez 

*F**ull-Stack DevOps Developer position at EMBL Grenoble*


EMBL Grenoble is looking for a Full-Stack Software Developer to join the 
Crystallographic Information Management System (CRIMS) project.


https://www.embl.org/jobs/position/GR00169

The Marquez Team operates the High Throughput Crystallization Laboratory 
(HTX Lab), one of the largest facilities of its kind in Europe and has 
developed automated pipelines for macromolecular crystallography and 
fragment screening based on the CrystalDirect technology. The web-based 
Crystallographic Information Management System (CRIMS) supports 
high-throughput operations at EMBL Grenoble, Hamburg and Heidelberg and 
multiple other laboratories in Europe supporting hundreds of scientists. 
CRIMS provides web interfaces for experimental design along with 
automated data capture from laboratory robots and sample tracking over 
the whole crystallography workflow, including communication with 
external data management resources at several Synchrotrons. The Current 
CRIMS team integrates three developers with different profiles.


We are looking for a dynamic and autonomous person, with a capacity to 
take initiatives to integrate and Lead the CRIMS software development 
Team under the supervision of the Head of the HTX Lab. You are a sharp, 
rigorous and methodical person with great problem-solving skills and 
have good interpersonal skills.


If you are interested in this position please apply here 
https://www.embl.org/jobs/position/GR00169


Best regards

Jose A. Marquez
**

--
_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/
https://htxlab.embl.fr/
_



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