Re: [ccp4bb] mutate Methionine to Norleucine in Coot.

Hi Xavier, Thank you for your suggestion. Bestt, Jiang On Wed, Oct 26, 2022 at 12:10 AM Xavier Brazzolotto wrote: > You can only link a residue (L-peptide) N to another residue (L-peptide) > N+1 or N-1 > In your case you try to link VAL10 to NLE101… > Just change NLE101 to NLE11, then it shou

Re: [ccp4bb] mutate Methionine to Norleucine in Coot.

Hi Paul, Thank you very much for your suggestions. It works. Sincerely, Jiang On Wed, Oct 26, 2022 at 3:22 AM Paul Emsley wrote: > > On 20/10/2022 02:06, Jiang Xu wrote: > > Hi Garib, > >I deleted the Met residue and imported the nle.cif file > > > Import the dictionary for NLE, make sure

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Re: [ccp4bb] Low resolution and high anisotropy

Dear Shenyuan Xu, I dealt with a similar problem recently. In my particular case, a combination of the correction with STARANISO and a subsequent molecular replacement with the MoRDa pipeline helped me a lot. MoRDa was able to suggest how to place individual protein domains separately in the u

Re: [ccp4bb] mutate Methionine to Norleucine in Coot.

On 20/10/2022 02:06, Jiang Xu wrote: Hi Garib,    I deleted the Met residue and imported the nle.cif file Import the dictionary for NLE, make sure that the group is L-peptide, then centre on your Met and Calculate -> Modelling -> Replace Residue -> NLE Paul. ##

Re: [ccp4bb] Low resolution and high anisotropy

How to deal with poor data is a challenge. Look at the images - see at what resolution there is detectable diffraction. Then run a self rotation function.Do you expect a trimer? dimer? etc and does the self rotation give any clues? Are your models dimers? trimers? etc. Eleanor On Wed, 26 Oct 2022

Re: [ccp4bb] mutate Methionine to Norleucine in Coot.

You can only link a residue (L-peptide) N to another residue (L-peptide) N+1 or N-1 In your case you try to link VAL10 to NLE101… Just change NLE101 to NLE11, then it should work. > Le 26 oct. 2022 à 03:37, Jiang Xu a écrit : > > Hi Garib, >Thank you for your reply. I edited the NLE.cif fil

Re: [ccp4bb] Low resolution and high anisotropy

Hello Shenyuan, I noticed STARANISO processd the data all the way down to 1.7A, with very poor stats on high resolution shell. I have two suggesstions: 1.You may want to reivse the raw images to check the anisotropy (that all spots are distributed in an flat ellipse). 2. If you can scale&mer