[ccp4bb] density modification

Hi CCP4ers firstly apologies that this is slighty off ccp4 topic but does anyone know of a programme whereby I can use solvent flipping with multi domain masks in addition to a solvent mask for my phases i.e. not based on a model? I wanted to use CNS density_modify (my maps are currently from

Re: [ccp4bb] Bacterial induction at 18C

Raji aside from the possibilites of toxic protein as already mentioned.. we had great results with overnight induction at 20oC for a protein that was somewhat insoluble at 37oC. One thing that might work for you is to grow the cells to OD 0.5 then lower the temperature to say 18 or 20.

Re: [ccp4bb] Concentrating protein

Hi there I quite like the Amicon stirred ultra concentration cell systems. You can put large volumes in, maximum 1 litre size, I think. As well you can attach an inert gas such as Argon or Nitrogen, for the gaseous pressure, this reduces oxidation of your sample while it concentrates.

[ccp4bb] Disordered domains in crystal strutures

Dear CCP4ers can anyone recommend papers describing crystal structures of proteins with a large functionally important disordered domain or domains. Thanks in advance Gina

Re: [ccp4bb] Concentrating protein

and Associate Consultant II Mayo Clinic Cancer Center Griffin Cancer Research Building, Rm 310 4500 San Pablo Road Jacksonville, FL 32224 (904) 953-6372 (office) (904) 953-0046 (lab) -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Gina Clayton Sent: Friday

[ccp4bb] Off topic question

Hi CCP4ers Apologies for the off topic... I have been trying to get hold of L-ribulose-5-phosphate (D- is readily available). Does anyone know where I can get such a compound? Thanks in advance for any suggestions and Merry Xmas! Gina

Re: [ccp4bb] more on lattice translocation defects

There is also another paper on this that may be useful... Acta Cryst. (2005). D61, 67-74. Correction of X-ray intensities from single crystals containing lattice-translocation defects. J Wang et al. Regards -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk]

[ccp4bb] Senior Crystallography Position

Posted on behalf of Professor John Hunt and Professor Liang Tong, North Eastern Structural Genomics Consortium at Columbia University USA. An accomplished and dynamic senior scientist is sought to join the high-throughput protein biochemistry / crystallography group of the Northeast Structural