First of all, thanks very much for those who replied to my question.
For those who do not follow this thread, I was asking a cryo question about
membrane protein crystals grown at 4C with 0.05-0.2M AS (ammonium sulfate) and
15-26%PEG400.
Some of you suggested directly freeze the crystal in LN
You didn't mention which detergent in the conditions. From the picture it looks
like crystals of detergent.
--
Hubing Lou
Centre for Biomolecular Sciences
University of St Andrews
North Haugh
St Andrews
Fife
KY16 9ST
Scotland
Quoting Ngo Duc Tri [EMAIL PROTECTED]:
Dear ccp4 users,
I
. 07920 138148
*From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of
*Hubing
Lou
*Sent:* 24 November 2010 14:09
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] unusual diffraction spots
Dear CCP4BBer,
I recently collected a dataset at synchrotron. The diffraction
Of
*Hubing
Lou
*Sent:* 24 November 2010 14:09
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] unusual diffraction spots
Dear CCP4BBer,
I recently collected a dataset at synchrotron. The diffraction was quite
anisotropic with one direction to 2.1Angstrom while the other is 3.0Ang.
What unusual
] *On Behalf Of
*Hubing
Lou
*Sent:* Thursday, July 21, 2011 6:46 AM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] Phaser and Molrep gave different solutions
Dear all,
I am stuck in a molecular replacement case and looking for advices.
I have been working on a protein-DNA complex structure.
Data
protein molecule or if the search models and the molecules have identical
sequences.
To solve this structure you probably have to do Se-labeled protein and SAD
etc. or collect anomalous from metal ions if present.
Cheers.
Ray Brown
--
*From:* Hubing Lou louhub
Dear all,
I have a structure solved by molecular replacement. The C-terminus at
the end of the chain shows as CO, not COOH. How do I make it into COOH
in COOT? I tried to place an O in the relevant position, but seems not
working this way.
Thanks,
Hubing
