Dear Crystallographers,
this is a sort of abstract question, but I think this listserve might have
some good answers.
In crystallography, we have a lot of nonlinearities or thresholds in
dealing with data. For example, the "atomic resolution" threshold, wherever
it be, defines whether we can use
Hi All,
haven't been following CCP4BB for a while, then I come back to this juicy
Holtonian thread!
Sorry for being more practical, but could you use a windowed approach:
integrate values of the same pixel/relp combo (roxel?) over time (however
that works with frame slicing) to estimate the
of the host. So why don't
> you work on the part of your conept over the week-end and present the
> concept?
>
> Cheers,
> Tim
>
>
> On Fri, 19 Feb 2021 12:55:32 -0500 Jacob Keller
> wrote:
>
> > I don't think seeing the big picture resolves, or even addr
n Pozharski
> > > > wrote:
> > > >
> > > >> I guess for such vehicle to be "extremely contagious" (or
> > > >> contagious at all for that matter) it should be capable of rapidly
> > > >> multiplying inside the
body to kick out the virus, and the cell that does not
> do it's job anymore, make you sick. A and B are mutually exclusive. B +
> C is named vaccine.
>
> Best,
> Tim
>
>
> On Wed, 17 Feb 2021 12:33:09 -0500 Jacob Keller
> wrote:
>
> > It would seem to me that it
exercised!
JPK
On Wed, Feb 17, 2021 at 2:29 PM David Schuller wrote:
> It was my understanding that you attended medical school. I am surprised
> that there was no instruction pertaining to the ethics of obtaining consent
> for human subject trials.
>
>
>
> On 2/17/21 2:09 P
ed, Feb 17, 2021 at 2:15 PM Philip D. Jeffrey
wrote:
> You're casting yourself in the Emma Thompson role for the remake of "I Am
> Legend" ?
>
> Phil
>
> --
> *From:* CCP4 bulletin board on behalf of Jacob
> Keller
> *Sent:
I was also thinking about vaccine distribution, how difficult it is.
The infectious vaccine is like lighting back-fires in forest fires.
One can certainly see why this would be instantly shot down by any pharma
CEO: all you need, theoretically, is enough for one infection, so negative
profit
But does it end better than the current best-seller "Smoldering Pandemic
Paralyzes Human Race, Fuels Contention, Kills Millions, Drives the Rest
Mad?"
JPK
On Wed, Feb 17, 2021 at 12:40 PM David Schuller
wrote:
> I read that book. It does not end well.
>
>
> On 2/17/21
It would seem to me that it should be possible to generate versions of the
Covid virus that would:
A. be extremely contagious and yet
B. be clinically benign, and
C. confer immunity to the original covid virus.
If, then, this virus could be released, with appropriate "kill switch"
safeguards
Is it infectious?
JPK
On Fri, Jan 29, 2021 at 2:14 AM Dale Tronrud wrote:
>
> Sometimes when explaining something, or even in private
> contemplation, it is nice to have a physical object as a point of focus.
> We, at the Corovavirus Structure Task Force, have created the design
> files
Brighter than XFEL? Or is it going to be an XFEL?
JPK
On Mon, Jun 3, 2013 at 10:36 AM, Derek Logan derek.lo...@biochemistry.lu.se
wrote:
Hi,
If anyone fancies becoming Life Science Director at the world's brightest
light source, look no further than here:
Can't you just show both cartoon (smoothed) and sticks (not smoothed) for
the given area?
JPK
On Thu, May 30, 2013 at 11:06 AM, Jason Vertrees
jason.vertr...@schrodinger.com wrote:
Hi Donghui,
Bernhard is correct: PyMOL flattens out secondary structure to produce
more aesthetically
Any thoughts of making a Windows executable? Might help a lot of users
JPK
On Wed, May 29, 2013 at 4:20 PM, Kay Diederichs
kay.diederi...@uni-konstanz.de wrote:
... is available for academic users at http://homes.mpimf-heidelberg.**
mpg.de/~kabsch/xds/
, May 29, 2013 at 1:29 PM, Jacob Keller
j-kell...@fsm.northwestern.edu wrote:
Any thoughts of making a Windows executable? Might help a lot of users
JPK
On Wed, May 29, 2013 at 4:20 PM, Kay Diederichs
kay.diederi...@uni-konstanz.de wrote:
... is available for academic users at http
Is this Laue diffraction in the sense that the neutrons are a spectrum of
energies, or does Laue mean something else here?
Jacob
On Thu, May 23, 2013 at 3:53 PM, Meilleur, Flora meille...@ornl.gov wrote:
IMAGINE, Neutron Crystallography Diffractometer
High Flux Isotope Reactor (HFIR),
Moreorless?
Dolores?
JPK
On Thu, May 2, 2013 at 12:22 PM, Eric Williams ericwilli...@pobox.comwrote:
I've always thought gormless is a woefully underused word.
Eric
On Thu, May 2, 2013 at 11:53 AM, Mark van Raaij mjvanra...@cnb.csic.eswrote:
i.e. the next program will be Graceless or
Nevertheless?
JPK
On Thu, May 2, 2013 at 2:18 PM, Roger Rowlett rrowl...@colgate.edu wrote:
Some of my undergraduate students need the CCP4 program feckless.
:)
___
Roger S. Rowlett
Gordon Dorothy Kline Professor
Department of Chemistry
Colgate
Never one to shrink from philosophizing, I wonder generally why the codon
conventions are the way they are? Is it like the QWERTY keyboard--basically
an historical accident--or is there some more beautiful reason? One might
argue that since basically all organisms share the convention (are there
keyboard configuration. I am dubious whether this model, however,
would apply to the codon conventions.
Jacob
On Tue, Mar 19, 2013 at 10:44 AM, David Schuller dj...@cornell.edu wrote:
On 03/19/13 10:34, Jacob Keller wrote:
Never one to shrink from philosophizing, I wonder generally why
Isn't lowering the symmetry equivalent to using multiple
models/conformations for one map? I remember seeing this done with the
infamous MSBA structure from a few years ago, so caveat emptor I guess. And
further, wouldn't using strict NCS make things equivalent to the
higher-symmetry space group?
that it is the best possible system, or
indeed should continue to be selected for (except that mutations to this
machinery tend to be very much deleterious).
-- David
On 19 March 2013 14:34, Jacob Keller j-kell...@fsm.northwestern.eduwrote:
Never one to shrink from philosophizing, I wonder
I don't understand this argument, as it would apply equally to all features
of the theoretical LUCA
No it won't. Different features would have different tolerance levels to
modifications.
Yes, this tolerance is the second (hidden or implicit) principle I
referred to. So you'd have to explain
Dear List,
Does anyone know of a source of quantification of membrane proteins in
garden-variety eukaryotic cell lines, e.g. HEK or HeLa cells (incidentally,
it just occurred to me that probably all HeLa cells are XX--seems right,
no?) I am looking for the highest-expressed, particularly, and
.
pdb1OKC). Single chain or homodimer, but apparently its not very easy to
crystallize.
Jacob Keller wrote:
Dear List,
Does anyone know of a source of quantification of membrane proteins in
garden-variety eukaryotic cell lines, e.g. HEK or
HeLa cells (incidentally, it just occurred to me
Well, wouldn't NCS be a parallel situation? I have heard, for example, that
the maps of viruses are considerably better at a given resolution than
monomeric proteins. So I would guess that someone has looked at this topic
in the case of NCS. Maybe high solvent content would be equivalent to
Did anyone see this prescient line in the PNAS paper? Seems that the MAD
concept was suggested way back then...
JPK
While the enhancement of anomalous scattering
has not yet been examined in detail, it is in principle
possible to use data collected at three wavelengths (15) to
completely solve
One final quote that is not in the twilight paper summarizes it nicely:
The scientist must be the judge of his own hypotheses, not the
statistician.
A.F.W. Edwards (1992) in Likelihood - An account of the statistical
concept
of likelihood and its application to scientific inference , p.
bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of
*Jacob
Keller
*Sent:* Tuesday, March 12, 2013 3:44 PM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* Re: [ccp4bb] validating ligand density
One final quote that is not in the twilight paper summarizes it nicely:
The scientist must
reasons for going for the gDNA.
Thanks again,
Jacob
On Tue, Mar 5, 2013 at 1:07 PM, Jacob Keller j-kell...@fsm.northwestern.edu
wrote:
Dear List,
please pardon the major off-topicity, but I need a trace amount of V
cholera genomic DNA to PCR from, and it just kills me to spend ~$300
Dear List,
please pardon the major off-topicity, but I need a trace amount of V
cholera genomic DNA to PCR from, and it just kills me to spend ~$300 to
order it for this one and only use. Is anyone in the DC/Baltimore area
willing and able to donate this? I travel on the capitol beltway daily, so
You only entertain addition+subtraction--why not use
multiplication/division to normalize the b-factors?
JPK
On Mon, Mar 4, 2013 at 2:04 PM, James Holton jmhol...@lbl.gov wrote:
Formally, the best way to compare B factors in two structures with
different average B is to add a constant to all
Along these lines, what reagents do people use to promote disuflide bonds,
i.e., the anti-DTT?
JPK
On Thu, Feb 28, 2013 at 2:06 AM, David Briggs drdavidcbri...@gmail.comwrote:
You might want to try Disulfide by design
http://cptweb.cpt.wayne.edu/DbD2/
Cheers
Dave
On Feb 28, 2013 6:55
Why not normalize each to its average b-factor? And...maybe they are not
significantly different in the first place?
JPK
On Mon, Feb 25, 2013 at 3:08 PM, Yarrow Madrona amadr...@uci.edu wrote:
Hello,
Does anyone know a good method to compare B-factors between structures? I
would like to
Dear Crystallographers,
it has been my experience that homology modelling programs get folds pretty
well, but sometimes the details are pretty obviously bad, like too-close
contacts. One might think that the modelling software would put in a sort
of polishing step, but they don't seem to. Is
I'd have to disagree on that. Protein crystals are fragile but not
soft. If your crystals are like gelatine it's unusual. It has been
demonstrated that elastic properties of protein crystals are similar to
organic solids
Interesting--do you have a reference quickly on hand for those
...@ufl.edu wrote:
**
The disulfide bond is intramolecular. I do not have reasons to believe it
is cross linking my protein
On Wed, 6 Feb 2013 22:16:36 -0500, Jacob Keller wrote:
Couldn't you just run reducing/non-reducing SDS-PAGE lanes and see the
difference?
JPK
On Wed, Feb 6, 2013
Couldn't you just run reducing/non-reducing SDS-PAGE lanes and see the
difference?
JPK
On Wed, Feb 6, 2013 at 11:10 AM, Yuri Pompeu yuri.pom...@ufl.edu wrote:
Dear All,
I am trying to probe the existence of a disulfide bond on the surface of
my protein.
I have attempted Ellman´s and my
Does that CA have a metal center (I think they all do)? If so, doesn't the
citrate compete for the metal?
JPK
On Mon, Feb 4, 2013 at 2:25 PM, Roger Rowlett rrowl...@colgate.edu wrote:
Human carbonic anhdyrase II can be easily crystallized from 1.3 M sodium
citrate/0.1 M TrisCl pH 8.5 at 10
Is anyone aware of any datasets taken at near absolute zero? I was
wondering what would happen...
Jacob
--
***
Jacob Pearson Keller, PhD
Postdoctoral Associate
HHMI Janelia Farms Research Campus
email: j-kell...@northwestern.edu
Were there really no computers in 1963?
JPK
On Fri, Jan 18, 2013 at 9:51 AM, David Schuller dj...@cornell.edu wrote:
http://eventheodd.blogspot.in/2013/01/golden-jubilee-of-ramachandran-plot.html
Golden Jubilee of Ramachandran
aided crystallization, so maybe it's
not so bad. Also, proteins often have salt in the protein stock as
well. Bottom line is that I wonder whether in Cale's case it might
have been the Na or the Cl that was the key, and perhaps not the
hepes/tris.
Jacob Keller
Dear all, thanks for your help--I made a very simple little script using
awk, and it works excellently (anyone is welcome to it, of course). Thanks
so much for taking the time.
Jacob
On Wed, Nov 7, 2012 at 9:49 PM, Jacob Keller j-kell...@fsm.northwestern.edu
wrote:
Dear Crystallographers
it
should be a very generally useful script to have around, and I need such
right now...
Thanks very much,
Jacob Keller
btw, this thread has one of my favorite titles ever...
JPK
On Tue, Oct 9, 2012 at 4:11 AM, Eleanor Dodson eleanor.dod...@york.ac.ukwrote:
This is interesting. In principle m and D should provide an optimum map,
and at high resolution they do a reasonable job.
The answer about occupancy is a
Alhtough perhaps a little more macabre--maybe you could crystallize
hemoglobin or myoglobin from blood or muscle (meat?). I recollect some
story of whale myo- or hemo-globin crystallizing on the salty decks of a
whaling ship...
Jacob
On Tue, Sep 25, 2012 at 11:59 AM, David Smith
Dear Crystallographers:
#off topic
I am trying to calibrate some microscopic imaging intracellular pH
measurements, but the usual nigericin (small pore-forming K+/H+ exchanger)
technique does not seem to be working well. Maybe it actually never works
well? Anyway, I was think of trying to use
For the gel, it might be really hard to see the peptide as small things
tend to blur terribly--better to look for shifts in the protein band. If I
were you, I would do serial dilutions of the peptide at some constant, very
visible protein concentration. Also you have to be sure that the
It turns out that the syntax and semantics of all reasonable programming
languages are very similar, or fall into only a few classes (e.g. C-like,
S-expressions, etc.), so once you are fluent in one from a class, it's
easy to pick up the others. This can't be said of natural languages, which
Dear List,
since this probably comes up a lot in manipulation of pdb/reflection files
and so on, I was curious what people thought would be the best language for
the following: I have some huge (100s MB) tables of tab-delimited data on
which I would like to do some math (averaging, sigmas, simple
For the specific purpose you list -
input from tab-delimited data
output to simple statisitical summaries and (I assume) plots
- it sounds like gnuplot could do the job nicely.
I wasn't aware that gnuplot can do calculations--can it? I was probably
going to use it somewhere as a plotting
Wouldn't bacteriorhodopsin be a good choice, especially since it's colored
and easy to express? Seems pretty expensive for a class, though, with all
the detergents involved...
JPK
On Tue, Sep 11, 2012 at 4:18 PM, Ho Leung Ng h...@hawaii.edu wrote:
Hello,
I am developing an
I know this isn't exactly your question, but it doesn't really take that
long to clone, express, and purify things nowadays--a few days, even? Also,
won't you be doing this anyway? So why not cut out the middle-man? Or,
better still, in your cloning downtime, do the software stuff.
JPK
On Thu,
How about just co-transfecting with two different plasmids which have
different selection markers? I've done it a lot, and it seems to work
fine...
JPK
On Fri, Aug 24, 2012 at 10:39 PM, Lye, Ming ming_...@hms.harvard.eduwrote:
Dear CCP4bb,
We would like to co-express proteins under Se-Met
if the
X-ray background is exactly zero and the observed (sic) intensity is
exactly zero.
--
*From:* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Jacob
Keller [j-kell...@fsm.northwestern.edu]
*Sent:* Thursday, August 23, 2012 12:36 PM
*To:* CCP4BB
Dear List,
I guess this is somewhat of a perennial issue, but I am faced with choosing
an OS for a new computer, and am curious about benefits and drawbacks with
regard to crystallography. So far, I have been using windows, and have
found no limitations whatsoever, but then again, maybe I don't
You mean cell in terms of a living cell, not a unit cell, right?
JPK
On Tue, Aug 7, 2012 at 10:24 AM, Paul Kraft haresea...@yahoo.com wrote:
Hi guys,
is there a program similar to ccp4/coot that allows one to visuallize an
ideal cell (either prokaryote or eukaryote) in 3D with semi accurate
Or similarly, the conditions in the crystallization drop (pH, ions, etc.)
may kick out the ligand.
JPK
On Fri, Jul 27, 2012 at 3:34 AM, herman.schreu...@sanofi.com wrote:
**
Dear Ni Shi,
Did you test a crystal, or uncrystallized protein? It may be that due to
crystal packing effects, only
Perhaps also exafs should be mentioned--I believe the various ion species,
redox states, and even binding geometry can be determined.
JPK
On Tue, Jul 24, 2012 at 12:37 PM, Roberts, Sue A - (suer)
s...@email.arizona.edu wrote:
Hello
Actually, if the home source uses a copper tube, neither
I think it's done on a crystal itself, but others who know better than I
can comment.
JPK
On Tue, Jul 24, 2012 at 1:02 PM, Theresa Hsu theresah...@live.com wrote:
Does EXAFS requires same amount of samples as ICP-MS/ICP-AES?
Theresa
On Tue, 24 Jul 2012 12:55:31 -0500, Jacob Keller
j-kell
How about trying some ARP/WARP?
JPK
On Wed, Jul 18, 2012 at 11:54 AM, Pavel Afonine pafon...@gmail.com wrote:
What happens if you do a round of refinement in phenix.refine after you
have done the mutations? Note: Phenix Autobuild is a tool to build your
model, not refine it (though it does
I was [too] obliquely alluding to this thread...
http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg27056.html
JPK
On Wed, Jul 18, 2012 at 12:32 PM, Edwin Pozharski epozh...@umaryland.eduwrote:
http://www.ysbl.york.ac.uk/ccp4bb/2001/msg00383.html
Rsym...what's that?
JPK
On
You probably already know this, but nitrogen is not at all poisonous--about
78% of the air is nitrogen. I guess you were probably worried about
asphyxiation?
JPK
On Fri, Jul 13, 2012 at 4:19 PM, Radisky, Evette S., Ph.D.
radisky.eve...@mayo.edu wrote:
Several have mentioned harvesting in
How frequently do the sensors go off?
JPK
On Fri, Jul 13, 2012 at 4:37 PM, David Schuller dj...@cornell.edu wrote:
On 07/13/12 17:29, Jacob Keller wrote:
You probably already know this, but nitrogen is not at all
poisonous--about 78% of the air is nitrogen. I guess you were probably
Give a list of all you have tried?
JPK
On Tue, Jul 10, 2012 at 12:22 PM, Muhammed bashir Khan
muhammad.bashir.k...@univie.ac.at wrote:
Dear All;
Could somebody give a nice suggestion how the following type crystal could
be optimized, I almost tried everything.
Crystal Image is attached
On Thu, Jul 5, 2012 at 1:44 PM, Eric Williams ericwilli...@pobox.comwrote:
I'm guessing disorder is a major limiting factor on effective resolutions.
;)
Now *that's* good...
JPK
On Thu, Jul 5, 2012 at 2:36 PM, Bernhard Rupp (Hofkristallrat a.D.)
hofkristall...@gmail.com wrote:
**Ø
First of all, isn't the choice either dimer or trimer, and second, as a
protein-detergent complex (PDC), it would be very unlikely that a trimer of
99 kD would run at 100 kD, although all is fair in love, war, and membrane
proteins.
JPK
On Thu, Jun 21, 2012 at 10:50 AM, Raji Edayathumangalam
): 01235 778664
--
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Jacob
Keller [j-kell...@fsm.northwestern.edu]
Sent: 21 June 2012 17:11
To: ccp4bb
Subject: Re
It seems to me that concentration is a statistical,
macroscopically-derived concept like temperature or pressure which
gets exceedingly weird when applied to microscopic phenomena. One
weirdness is, I guess, that the somewhat arbitrary size of the box you
mentioned makes a huge difference in the
What extra insight does the full-length protein give, i.e., why not
just chuck it?
JPK
On Tue, Jun 19, 2012 at 10:35 AM, RHYS GRINTER
r.grinte...@research.gla.ac.uk wrote:
Dear All,
I'm working out the finer details on a structural paper for submission to
JBC. I'm having a slight problem
Is part of your question based there being many ways to superimpose
structures, which there are?
JPK
On Tue, Jun 19, 2012 at 5:34 PM, Petr Leiman petr.lei...@epfl.ch wrote:
Would anyone be kind enough to explain what kind of information does the
RMSD for two not-yet-superimposed structures
Wow, that's very cool! Can you divulge what the function of the
protein is? One thinks of some kind of mechanical spring...
JPK
On Mon, Jun 18, 2012 at 8:49 AM, anna anna marmottalb...@gmail.com wrote:
Hi all!
I'd like your opinion about a structure I solved.
Apart from protein structure
I have been curious and suspicious for a long time about multimer: I
always assumed it to be a more homey substitute for oligomer, as
there seems to me to be no difference in usage, and certainly not in
the etymological sense. I have often heard it used by non-experts who
don't know exactly the
I love myriomer, but what's wrong with boring old polymer?
JPK
On Mon, Jun 18, 2012 at 10:27 AM, Emmanuel Saridakis
esari...@chem.demokritos.gr wrote:
Of course, oligomer (pure Greek) usually does that kind of job, but not in
this specific case, since oligo means few and in this case we have
at 10:37 AM, David Schuller dj...@cornell.edu wrote:
On 06/18/12 11:17, Jacob Keller wrote:
But anyway, what is
wrong with calling her structures polymers? Is there a subtle
covalent insinuation to polymer?
subtle? No, it's not subtle
I suspect that there was a time when the anomalous signal in data sets was
fictional.
Before the invent of flash freezing, systematic errors due to decay and the
need
of scaling together many derivative data sets collected on multiple crystals
could render
weak anomalous signal useless.
I think some have used anomalous signals since the 1930s-40s, e.g., Bijvoet!
JPK
On Wed, Jun 6, 2012 at 10:23 AM, Ronald E Stenkamp
stenk...@u.washington.edu wrote:
There were a number of labs using anomalous dispersion for phasing 40 years
ago. The theory for using it dates from the 60s.
...Even with such primitive techniques, I can remember an HgI4
derivative in which you could safely refine the anomalous occupancies
(i.e. f values) for the iodine atoms of the beautiful planar HgI3 anion to
5 electrons.
I am surprised--f's of I and Hg are supposed to be around 8 for CuKa
(or
) might be an alternative and
perhaps more enlightening way of getting a picture of the evolution of
phasing methods than finding some clever filter settings in the RCSB ;-) .
With best wishes,
Gerard.
--
On Wed, Jun 06, 2012 at 11:08:37AM -0500, Jacob Keller wrote:
...Even
-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob
Keller
Sent: Wednesday, June 06, 2012 11:30 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fun Question - Is multiple isomorphous replacement an
obsolete technique?
No offense taken (we all have our dour moments
I don't think any data should be discarded, and I think that although
we are not there yet, refinement should work directly with the images,
iterating back and forth through all the various levels of data
processing. As I think was pointed out by Wang, even an intensity of 0
provides information
Let's say you collect data (or rather indices) to 1.4 Ang but the real
resolution is 2.8 Ang and you use all the data in refinement with no
resolution cut-off, so there are 8 times as many data. Then your 15
mins becomes 2 hours - is that still acceptable? It's unlikely that
you'll see any
Meant to include the following link in the previous message:
http://www.youtube.com/watch?v=9Jn8K8EA7-Q
JPK
On Thu, May 31, 2012 at 1:20 PM, Jacob Keller
j-kell...@fsm.northwestern.edu wrote:
Dear Crystallographers,
in case you have not heard, it would appear that the Rmerge statistic
has
of this program RESCUT.
REMARK 200 R SYM FOR SHELL (I) : 1.21700
I noticed structure 3RKO reported Rmerge in the last shell greater
than 1, suggesting the police who were defending R-merge were fighting
a losing battle. And this provides a lot of ammunition to those
they are fighting.
Jacob
Good idea, but how to get it to catch on without publishing in Science?
JPK
On Thu, May 31, 2012 at 4:21 PM, Dale Tronrud det...@uoxray.uoregon.edu wrote:
On 05/31/12 12:07, Jacob Keller wrote:
Alas, how many lines like the following from a recent Science paper
(PMID: 22605777), probably
I believe that's an alternative conformationtry modeling as such and
see what happens.
Jacob
On Mon, May 21, 2012 at 3:57 PM, Uma Ratu rosiso2...@gmail.com wrote:
Dear All:
Some of serine residues in my model have extra positive Fo-Fc density at
the edge of side chain. Some don't have.
So much easier is to simply loop your favorite tubing a couple of times
through a small N2 dewar, then direct the output into a 15mL tube immersed
in same. Or, simply point the tubing directly into the immersed tube, and
flow slowly. In both cases, the cold N2 will condense the propane. Of
course,
Well, not to be a downer, but wikipedia has some comments on the hazards...
Environmental effects
Tetrafluoromethane is a potent greenhouse
gashttp://en.wikipedia.org/wiki/Greenhouse_gas that
contributes to the greenhouse
effecthttp://en.wikipedia.org/wiki/Greenhouse_effect.
It is very stable,
Maybe it's including covert structure factors? See recent ccp4bb post
subject...
JPK
On Tue, May 15, 2012 at 4:28 PM, case c...@biomaps.rutgers.edu wrote:
On Tue, May 15, 2012, Toth, Eric wrote:
In sports, maximal effort is considered to be 110%, so you're actually
9.9% short of getting
How about chloride? I know, there are negative electrostatics, but I think
such is seen sometimes for halides. Or, is it possible that there is a side
chain flipped?
Jacob
On Tue, May 15, 2012 at 9:51 AM, RHYS GRINTER
r.grinte...@research.gla.ac.uk wrote:
Dear Community,
As I'm a relatively
Try adding water first, so that you are not mixing concentrated Zn with
concentrated HEPES. Also it depends what else is in your cocktail.
JPK
On Fri, May 11, 2012 at 11:26 AM, Rajesh Kumar ccp4...@hotmail.com wrote:
Dear All,
This question sounds simple but I dont know the answer.
I was
Just to make sure I understand pH correctly: isn't it true that the [OH-]
should always be the same at a given pH (by definition)?
JPK
On Fri, May 11, 2012 at 11:48 AM, Katherine Sippel
katherine.sip...@gmail.com wrote:
That is probably because you pH Tris with HCl rather than HEPES with
mitegen loops might help, particularly micromesh...
JPK
On Fri, May 11, 2012 at 12:35 PM, Rajesh Kumar ccp4...@hotmail.com wrote:
Dear Patrick,
You along with others had made some suggestions last time. May be its a
good time to update.
With classical screening, I got a crystal like
Dear Crystallographers,
the saxs on crystals thread reminded me of a question I have had for a
while, and never having collected data better than ~1.6 Ang or so, cannot
answer myself from experience: I would think that there might be
powder-like diffraction rings at distances corresponding to the
I saw something online about the EIGER 16M: 201 GB of data per second! Is
that number correct?
JPK
On Wed, May 9, 2012 at 9:58 AM, Meitian Wang meitian.w...@psi.ch wrote:
Postdoctoral Fello*Next Generation Detector for Protein Crystallography*
Your tasks
Built on the success of PILATUS
interference.
Tim
On 05/09/12 16:16, Jacob Keller wrote:
Dear Crystallographers,
the saxs on crystals thread reminded me of a question I have had
for a while, and never having collected data better than ~1.6 Ang
or so, cannot answer myself from experience: I would think that
there might
Yes, I just looked up the paper--seems right on topic--a powder-type ring
at ~4.2 Ang, corresponding to Calpha-Calpha distances! But no 1.2-1.5 Ang
ring, from what I saw. Maybe it gets swamped out by other things. I am
thinking that the variety/distribution of bonds/distances of length 1-3 Ang
in
It seems to me that spherical forms of Wilson plots could be used to
determine how many bonds of what nature were oriented in which direction,
and this may have been what Bricogne's micro molecular replacement
technique was capitalizing on? For example, one might be able to orient a
straight DNA
Dear Colin,
the table you gave seems to have been from Fe2+Fe3+2O4 or from Fe3-xTixO4.
I am curious what the nature of the Fe inside the ferritin is (I don't
think it has Ti in it, though...). Is it elemental iron?
Also, to Anna: can you send a picture of that diffraction pattern with spot
It might be that the bulk solvent correction is nullifying the interior
of the ferritin structure, and there should be a way to tell the refinement
software not to treat the interior as solvent. Perhaps then you might find
your Fe? Also, I would think there should be some powder-like diffraction
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