[ccp4bb] Use MAPMAN to transform spacegroup of an EM map from P1 to P4

Hi, I have an EM map of a tetrameric protein. It was painful to work with this map since it is in P1 spacegroup, although 4-fold symmetry was already applied during map reconstruction. I noticed that people used MAPMAN to transform spacegroup, however, it seems not working for me. The map

[ccp4bb]

> map and have only symmetry independent copy to work with until finishes, > then make the whole molecule. For real-space refinement it's totally > irrelevant whether you have whole molecule or 1/Nth of it. So.. it isn't > clear what the problem is.. > Pavel > > On Thu, May 18,

[ccp4bb]

y why can't you simply build your model > once in one quarter of the map? What do you hope to change by > specifying that the space group is P4? > > Dale Tronrud > > On 5/18/2017 10:06 PM, Qingfeng Chen wrote: > > Hi, > > > > I have an EM map of a tetrameric prot

[ccp4bb]

Hi, I have an EM map of a tetrameric protein. It was painful to work with this map since it is in P1 spacegroup, although 4-fold symmetry was already applied during map reconstruction. I noticed that people used MAPMAN to transform spacegroup, however, it seems not working for me. The map

[ccp4bb]

Thanks for the suggestion about using NCS constraint to apply change on one subunit to other subunits. That should make life much easier. About the problem of phenix realspace refinement, I figured it out. I used GUI and when I checked the log file, the "ramachandran_restraints" was turned off.