Dear Gourab,
You might just want to add the ligand in pure, solid form to the edge of the
crystallization drop. This worked in our case beautifully (see Angew. Chem.
Int. Ed. 2012, 51, 3354). We had to soak it for 4 weeks.
Good luck!
Best regards
Bernhard
questions. You will find descriptions about
different crystallization techniques and which (organic) solvents you can use
for a start.
If you have further questions, just send me an email.
Best regards
Bernhard
Prof. Dr. Bernhard Spingler
Department of
Dear ccp4bb members,
I would be most happy, if you could point me to any publications, in
which results of crystal annealing by warming up a
DNA/RNA/oligonuculeotide crystal and again cooling it down to low
temperatures were reported. I am aware of 2 publications that discuss
this for
