You need to have crystal between polarizing plates. If you use only one on the
top (close to your eye) and rotate filter you will “filter/polarize” all the
light coming to your eye. What you need to buy is polarizing filter sheet
(linear) which you place under your crystal and another one (the
Hi Alan,
I think new AKTA collector can be refrigerated. If this is not an option,
consider putting only collector in the cold cabinet and cooling down the
buffers. Also, you can put only columns and fraction collector inside cabinet
which has side port for such use. Although you will have to
I have noticed it with new pucks (most likely some kind of grease from
manufacturing) and later on when used in dry shipping dewars that has old
absorbent coating. Also it might be that your dewar is not holding vacuum very
well. Usually it can be cleaned with compressed air but have decided
,
Michal Boniecki
--
Michal T. Boniecki, Ph.D.
University of Saskatchewan
Department of Biochemistry, Microbiology and Immunology
Manager Protein Characterization and Crystallization Facility
Health Sciences Building 3D40
107 Wiggins Rd.
Saskatoon, SK S7N 5E5
Canada
tel.(306)966-6307
In my case what simply worked is to put your solution with 40% glycerol on the
side of the drop and wait for crystals to stop dancing. Fish them out swiping
through the side were you have put your solution. Or I have used MPD as
cryoprotectant and freeze them in cold room (4C) its amazing what
