Re: [ccp4bb] Native Gel Theory and Practice

the way to go. Nat Protoc. 2006;1(1):418-28. Blue native PAGE. Wittig I, Braun HP, Schägger H. best, hannes uchtenhagen Hannes Uchtenhagen Karolinska Institutet Center for Infectious Medicine (CIM) Karolinska University Hospital Huddinge, F59 SE-141 86 Stockholm, Sweden Office: +46-(0)8-524 86981

[ccp4bb] smeared bacteria pellets in protein expression

could cause this kind of problem (IPTG, aeration, leftovers from glassware cleaning, media) or if I missed something with respect to the phages. Many thanks for your time! hannes uchtenhagen -- Hannes Uchtenhagen Karolinska Institutet Center for Infectious Medicine (CIM) Karolinska University

Re: [ccp4bb] Off-topic: chemical modification on thiol groups

Dear Remy, Iodoacetamide might be a good candidate as it alkylates thiol groups and is also used as cysteine protease inhibitor. good luck, hannes - Hannes Uchtenhagen, Ph.D student Karolinska Institutet Center for Infectious Medicine (CIM) Karolinska University Hospital Huddinge, F59 SE-141