[ccp4bb] off topic: Add ligands/substrate directly into expression medium?

around 0.5-1mM. Can i add sterols directly to the medium , when i expression my protein ? If not , then in which condition can we do like this, for inhibitor ? i remeber i read one paper did like this. Sincerely! Liuqing chen

[ccp4bb] the purification process of protein, used for crystallization

step, why not the SEC used at the finally step? sincerely Liuqing Chen

[ccp4bb] crystallization optimization

hello everyone! I get a condition (10% w/v PEG 6000, 100mm HEPES PH7.0) in which my protein grow small needle like crystals, how can i optimize it to get bigger crystals? the attach is the crystals figure. thanks in advance sincerely Liuqing Chen

[ccp4bb] prepare stock solution of hampton heavy atom screen kits

Hello everyone! I want to prepare heavy atom stock solution to soaking my crystal, what should i use to soluble the heavy atom Pt, Hg and other hampton heavy atom screen kits, as i know someone not good soluble in water? can anyone give me some ideas! sincerely Liuqing chen

[ccp4bb] Help with MR result

/warp， it's built model worse. I don't know what to do? can anyone give me some suggestions? Thanks Liuqing Chen