Re: [ccp4bb] Cysteine-S-Iodine interaction

[Michał Taube]

Hi,
it may be oxidized cysteine. Look here:
https://www.researchgate.net/publication/44656220_Protein_S-thiolation_by_glutathionylspermidine_Gsp_The_role_of_Escherichia_coli_Gsp_synthetASEamidase_in_redox_regulation

and here:
Trundle D, Cunningham LW. Iodine oxidation of the sulfhydryl groups of
creatine kinase. Biochemistry. 1969;8:1919–1925.

Best Michal

czw., 17 mar 2022 o 18:37 Mohd Syed Ahangar 
napisał(a):

> Hi CCP4BB members.
>
> I have been doing some protein crystal soaking with some covalently
> binding fragments and in one structure I have got an extra density on
> Cysteine but that density doesn't match with the expected fragment. The
> fragment was in the form of iodide salt. when I fit the Iodine in the
> density, it fitted fairly well than any other possible chemical entity.
> From the density map it looks like something is covalently bound to
> Cysteine.
> Now my question is, can a sulphur atom of Cysteine have such an
> interaction with Iodine. The distance between S and Iodine is 2.76A in this
> case as shown in the attached figure.
> I would be grateful if someone can shed some light on this.
>
> Thank you in advance.
> --
>
> Dr. Mohd Syed Ahanger,
>
> Faculty of Biological Sciences,
>
> University of Leeds, Leeds, UK.
>
> --
>
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Re: [ccp4bb] Off-topic x 2: Chaperone co-expression AND Bac-Mam contract work

[Michał Taube]

Hi,

You can find GroESL expressing plasmid and plasmids with different
chaperones combinations from prof. Bernd Bukau lab on Addgene:

https://www.addgene.org/Bernd_Bukau/

Best
Michal

2017-01-20 3:19 GMT+01:00 Patrick Loll :

> Hello everyone,
>
> Please allow me to kill two off-topic birds with one email ‘stone’:
>
> 1) Can anyone suggest where I can lay hands on the pREP4-GroESL plasmid
> for bacterial co-expression of GroEL and GroES? I’ve checked with the
> original author (Philip Cole), but it’s been decades since he published on
> this, and he’s not sure he can find the plasmid. However, I see recurrent
> (and recent) references to the plasmid in the literature, so I guess that
> it’s loose in the wild…
>
> 2) We’re looking for a contract research organization to do some Bac-Mam
> expression for us (we’re in a hurry to get some results, so we’d like to
> farm it out rather than get the technology going in-house). Can anyone
> recommend a group (ideally in the US, for logistical ease)?
>
> Thanks for any help.
>
> Cheers,
>
> Pat
>
>
>
> 
> ---
>
> Patrick J. Loll, Ph. D.
>
> Professor of Biochemistry & Molecular Biology
>
> Director, Biochemistry Graduate Program
>
> Drexel University College of Medicine
>
> Room 10-102 New College Building
>
> 245 N. 15th St., Mailstop 497
>
> Philadelphia, PA  19102-1192  USA
>
>
> (215) 762-7706
>
> pjl...@gmail.com
>
> pl...@drexelmed.edu
>
>