Re: [ccp4bb] [OFF TOPIC] On Ni-NTA resin TEV cleavage

Hi Rafael, the simple answer is that the EDTA and DTT( or any other reducing agent) is not required for the TEV activity. You can have your TEV protease in 20 mM Tris pH 7.5 (RT) and 150 mM NaCl, plus either 10% Glycerol or 50% glycerol depending on storage conditions preference - 80 or -20C

Re: [ccp4bb] Tagging a membrane protein after the Signal peptide

on the WCL, but not on Flow cytometry as it is not represented on the surface. I hope that helps, Best wishes Nikolay Dobrev On Thu, Jun 22, 2023 at 11:57 AM samer halabi <30c2162795b2-dmarc-requ...@jiscmail.ac.uk> wrote: > > Dear All, > > Sorry to be disturbing you all with

Re: [ccp4bb] TEV vs HRV3C

my head. I hope they are helpful and wish everyone happy cleaving of the fusion proteins :) Kind regards, Nikolay Nikolay Dobrev Postdoctoral Fellow @ Wilmanns group EMBL Hamburg, c/o DESY, Building 25A, Notkestraße 85, 22607 Hamburg, Germany T +49 40 89902 165 | M +49 173 684 0532 twitter.

[ccp4bb] Research Technician position (HH00211) @ EMBL Hamburg

/HH00211 Kind regards, Nikolay Nikolay Dobrev Postdoctoral Fellow @ Wilmanns group EMBL Hamburg, c/o DESY, Building 25A, Notkestraße 85, 22607 Hamburg, Germany T +49 40 89902 165 | M +49 173 684 0532 twitter.com/emblevents https://twitter.com/emblevents |http://facebook.com/embl.org | http

Re: [ccp4bb] Off topic - gene toxic for expression strains

approach. Let me know if you need any further tips. Nikolay Dobrev Postdoctoral Fellow @ Wilmanns group EMBL Hamburg, c/o DESY, Building 25A, Notkestraße 85, 22607 Hamburg, Germany T +49 40 89902 165 | M +49 173 684 0532 twitter.com/emblevents https://twitter.com/emblevents |http://facebook.com

Re: [ccp4bb] [External] Re: [ccp4bb] protease inhibitor and 3c protease

gt; Thank you > Dhiraj > > > - > From: CCP4 bulletin board on behalf of Nikolay > Dobrev > Sent: Thursday, February 3, 2022 11:53 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [External] Re: [ccp4bb] protease inhibitor and 3c protease > > Dear Dhi

Re: [ccp4bb] protease inhibitor and 3c protease

; To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > Nikolay Dobrev Postdoctoral Fellow @ Wilmanns group EMBL Hamburg, c/o DESY, Building 25A, Notkestraße 85, 22607 Hamburg, Germany T +49 40 89902 165 | M +49 1

Re: [ccp4bb] Searching Sequence Motif

MG1655 Cannot be 100% sure if you select PDBSTR, if it will really use the PDB database, but it is worth trying. you can look up the list for all organisms here: https://www.genome.jp/kegg/catalog/org_list.html Best wishes, Nikolay Nikolay Dobrev Postdoctoral Fellow @ Wilmanns group EMBL

Re: [ccp4bb] protein oligomer

observed different specises when you analyze your protein by SEC or if you are able to perfom DLS? Additional information regarding your protein will be really helpful for more detalied suggestions how to overcome your protein. Best, Nikolay Nikolay Dobrev Scientific Officer, Protein Expression

[ccp4bb] Open position: Scientific Officer - Biophysical Characterization at EMBL Heidelberg

/searchjobs/index.php?ref=HD01727=1[]=1 (https://www.embl.de/jobs/searchjobs/index.php?ref=HD01727=1[]=1) Best Regards,Nikolay Dobrev Nikolay Dobrev Scientific Officer, Protein Expression and Purification Core Facility EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany T +49 6221 387 8633 | M

Re: [ccp4bb] CCP4BB vs COVID19

/pathogens/covid-19) You can find all relevant data, from COVID-19 genome sequencing data up to x-ray and cryo-EM structures of relevant proteins. Stay healthy, Nikolay Nikolay Dobrev Scientific Officer, Protein Expression and Purification Core Facility EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg

[ccp4bb] Open position: Scientific Officer at PepCore EMBL Heidelberg

! Closing date: 7 November 2019 Link to the job add: https://www.embl.de/jobs/searchjobs/index.php?ref=HD01639=1[]=1 (https://www.embl.de/jobs/searchjobs/index.php?ref=HD01639=1[]=1) Best Regards, Nikolay Dobrev Nikolay Dobrev Scientific Officer, Protein Expression and Purification Core Facility

[ccp4bb] Anderson?Evans polyoxotungstate (TEW)

/diffraction? Any comments will be highly appreciated. Nikolay Dobrev Nikolay Dobrev PhD Student in AG Sinning & AG Fischer Biochemie Zentrum Heidelberg University Im Neuenheimer Feld 328 69120 Heidelberg Germany Phone: +49 6221 54