[ccp4bb] molprobity

2013-11-11 Thread SD Y
Hi, I was wondering if molprobity server is down (http://molprobity.biochem.duke.edu/index.php) or its just my files or our network has problem because I cant upload my files (some times it gave error). ThanksSDY

Re: [ccp4bb] molprobity

2013-11-11 Thread SD Y
. On Nov 11, 2013, at 10:02 AM, SD Y wrote: Hi, I was wondering if molprobity server is down (http://molprobity.biochem.duke.edu/index.php) or its just my files or our network has problem because I cant upload my files (some times it gave error). Thanks SDY John J. Tanner

Re: [ccp4bb] low-resolution and zinc

2012-11-09 Thread SD Y
and Biotechnology, Department of Molecular Microbiology and Biotechnology Tel Aviv University 69978, Israel Acta Crystallographica F, co-editor e-mail: mbfro...@post.tau.ac.il Tel: ++972-3640-8723 Fax: ++972-3640-9407 Cellular: 0547 459 608 On Nov 9, 2012, at 20:09 , SD Y ccp4...@hotmail.com wrote:Dear

[ccp4bb] low-resolution and zinc

2012-11-07 Thread SD Y
Dear all, I have a related question to the one I have posted low resolution and SG, on which I am still working based on the suggestions I have got. The model I have used, has Zn co-ordinated well in tetrahydral fashion by 3 cys and 1 His residues. They have add Zn in to their experiment.

Re: [ccp4bb] protein cleavage

2012-11-04 Thread SD Y
Rana, I understood that these proteases are very efficient in cleavage. One time, I had a construct MBP-3C-protein, I never able to cleave this particular construct while I could do well with other truncations.The MBP you are seeing might be co-purified with DHBx and unless gel band intensity