Re: [ccp4bb] Fab:antigen complex crystallization!!!

2011-07-28 Thread Alexandre Wohlkonig
Hi Ivan, Here is another example of a method to crystallize antibody/antigene complexes. It uses a limited proteolysis step to generate crystals of poor quality, which are then used as seeds for an MMS screening... http://www.ncbi.nlm.nih.gov/pubmed/21536542 Good luck, Alex 2011/7/28

Re: [ccp4bb] protein lost activity after size exclusion chromatography

2011-03-16 Thread alexandre wohlkonig
Hi Harvey, Could it be that the activity you're measuring comes from a contaminant? Did you test the other fractions from SEC or IEX? Cheers, Alex 2011/3/16 Harvey Rodriguez h.rodriguez.x...@gmail.com Dear all, Recently, I came across an obstacle on the purification and acitivty