Dear all,
I have a protein with molecular weight of 45 kDa which is part of a
multisubunit complex. I was able to crystallize it, which diffracted to
resolution of 8 Angstroms. I would like to increase the resolution of the
protein crystals by trying to keep the protein intact with less possible
t
Dear all,
I have problems in purifying a protein. The protein is 38,000 daltons and
has a N-ter His-Tag. The protein expression levels are low and as a result I
have a limit for the purification steps.
Initially I used NiNTA columns with 50 mM sodium phosphate buffer pH8, 300
mM NaCl, 20 to 250 mM
