I would disagree. You list as sequence what you cloned and expressed. If you
are missing parts then you have to describe why e.g. Proteolysis or disordered
etc.
Best to actually do a mass spec analysis on those crystals since a significant
portion is missing.
Jūrgen
..
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry Molecular Biology
Johns Hopkins Malaria Research Institute
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http://lupo.jhsph.eduhttp://lupo.jhsph.edu/
On Dec 30, 2014, at 15:50, Jeffrey, Philip D.
pjeff...@princeton.edumailto:pjeff...@princeton.edu wrote:
Mohamed,
You always list the sequence of what's actually in the crystal, e.g. 1-105.
(Not: what's in the model or what the sequence of the full length protein is).
Make sure that if there's any lingering residues from any affinity/purification
tags they get included in the sequence too.
Phil Jeffrey
Princeton
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK]
on behalf of Mohamed Noor
[mohamed.n...@staffmail.ul.iemailto:mohamed.n...@staffmail.ul.ie]
Sent: Tuesday, December 30, 2014 3:26 PM
To: CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] PDB deposition - sequence file
Dear all
The protein that was crystallized is only the first 105 residues of a
230-residue protein. In the structure, I can see density for residues 6-72. For
deposition, should the whole native/biological sequence be deposited?
Thanks.
Mohamed
