Dear all,
Sorry for the non-crystallographic question. Currently I am working on a
zinc binding protein which is expressed in insect cells and may contain 4-6
zinc ions. As we know, so many zinc binding proteins can absorb the iron
ions from the culture medium and the protein looks from yellow to
Hi Heng,
DTT can react with metal cations such as Zinc or Iron. This is why people
to tend to use little DTT or no DTT at all on metal affinity columns or
replace it b-mercaptoethanol or TCEP that do not interfere.
Regarding the incorporation of Zinc into the culture media.
I recall that Zinc
Harvey,
Depending on the zinc-binding site, it may not bind Fe(II) at all.
Zn(II) and Fe(II) have very different preferred ligand binding
environments. For many zinc-metalloenzymes, substitution with Fe(II)
would be difficult to impossible. In general, you will find it very
difficult to make
