Dear Community,
In trying to trouble shoot an experiment I have become interested in
the cellular process that regulates the insertion and proper
orientation of membrane proteins. I am looking for references for how
a GPCR is correctly oriented during expression (i.e. the extra
cellular
Hi Justin,
I'm not sure if there are papers regarding this for GPCRs, but the phenomenon
you're referring to is the positive inside rule. This basically means that
the SecY translocon (in a way that is only partially clear) mediates membrane
protein insertion in such a way that the (net)
Hi Justin,
Since GPCRs are polytopic a-helical transmembrane proteins, it is very
likely that (1) insertion into the membrane is primarily performed by the
Sec61 complex AKA translocon and (2) targeting to the membrane would be
controlled by the signal recongition particle and its receptor. the
In addition to Bert remarks, you can read this paper from the positive
inside rule instigators.
Seppälä S, Slusky JS, Lloris-Garcerá P, Rapp M, von Heijne G. Control of
membrane protein topology by a single C-terminal residue. Science. 2010
Jun 25;328(5986):1698-700. Epub 2010 May 27. PubMed
