Hi Francis
I guess it depends on how much residual high-affinity binder you have in the
mixture and what the difference in affinity is between Y and deriv-Y. Another
issue is of course whether Y and derY compete for the same binding site and
have the same stoichiometry. A well designed
Hi Francis,
I might save you some time by telling you up front you should just go
back and purify your compound to remove the impurity, you dont even
need to read the rest of this, just go.
Along the lines of what Savvas was saying, with any equilibrium
binding assay between two direct
Hi All
I'm curious the effect of small impurities in commercially synthesized
compounds on ITC and its analysis. Say if compound Y is the high
affinity binder, but you make a derivative that differs from a single
functional group from Y (you used Y to make this new compound) and you
the influence of Y component.
I hope this give the slightest hint!!
Ahmed.
--- On Tue, 8/24/10, Francis E Reyes francis.re...@colorado.edu wrote:
From: Francis E Reyes francis.re...@colorado.edu
Subject: [ccp4bb] offtopic: effect of compound impurities on ITC?
To: CCP4BB@JISCMAIL.AC.UK
Date: Tuesday
