Dear Friends,
I recently have a partially complete(90%) model that
refined to R = 26%
and R(free) = 30% at 2.5 A data and looks fine in the 2fo-fc map. This is a
complex molecule comprising two proteins and one
dna in the AU. Protein 1(285 aa) and dna(15 base pairs) have been solved and
refined well. Missing 10 % is protein 2 containing 45 aa. In the 2fo-fc map
created from the partial model(without protein 2) I can see some density for
the missing protein 2.
I tried Phaser and Molrep in ccp4 fixing the partial model to solve the missing
protein 2, but as of no success. The density for the missing part in the 2fo-fc
map
is not good enough for manual building. The missing protein 2 contains a Zn
finger where the Zn atom is co-ordinated by 4 S atoms. I am wondering if
someone can suggest
me a good way to develop the missing part from this point.
Thanking you in advance for your suggestions…
Raja
Add more friends to your messenger and enjoy! Go to
http://messenger.yahoo.com/invite/
