Hi Firdous, What Diana suggested may work if done carefully; however, I failed to transfer crystals from 1.5 LiSO4 salts to higher concentrations of LiSO4 in many attempts. I did have success in transferring said crystals to 1.5 M sodium malonate then to incrementally to 2.8 or 3.2 M sodium malonate.
Best regards, Blaine Blaine Mooers, Ph.D. Associate Professor Department of Biochemistry and Molecular Biology College of Medicine University of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center (BRC) Rm. 466 975 NE 10th Street, BRC 466 Oklahoma City, OK 73104-5419 office: (405) 271-8300 lab: (405) 271-8313 e-mail: blaine-moo...@ouhsc.edu <mailto:blaine-moo...@ouhsc.edu> alternate e-mail: bmooe...@gmail.com <mailto:bmooe...@gmail.com> Faculty webpage: https://tinyurl.com/BMBmooers Google Scholar: https://tinyurl.com/GSbmooers SSRL User Executive Committee: https://www-ssrl.slac.stanford.edu/content/about-ssrl/advisory-panels/ssrl-users-organization SSRL-LCLS User Meeting: https://conf.slac.stanford.edu/ssrl-lcls-2018/ EasyPyMOL: https://github.com/MooersLab Molecular Graphics Course Links: https://tinyurl.com/molgr Small Angle Scattering Links: https://tinyurl.com/ouhscsaxs X-ray lab (LBSF): https://tinyurl.com/ouhsclbsf ________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Firdous Tarique [kahkashantari...@gmail.com] Sent: Friday, October 19, 2018 4:57 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [EXTERNAL] [ccp4bb] suggestions for cryoprotectant Dear members I have got beautiful crystal hits in SaltRx screens which are not diffracting to a good resoultion. All of them are salt based condition and I am not able to formulate a good cryoprotectant for these crystals. I also think that in my case the poor resolution is due to a poor cryoprotectant selection. The conditions are as follows: 1> 4M Ammonium Acetate 100mM Bis Tris Propane pH 7.0 2>0.5M KCN 100mM Tris pH8.5 3>1.5M LiSo4 100mM Bris Tris Propane pH 7.0 4>4M Sodium Nitrate 100mM Tris pH8.5 5>1.5M Sodium Nitrate 100mM Sodium acetate pH 4.6 There are few more conditions but so far not able to see good diffraction with using lower peg and glycerol based cryoprotectants. Can anybody suggest me good cryos conditions for salt based crystallization conditions or anything good for SaltRx crystallization hits. Thanks Firdous ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=VjzId-SM5S6aVB_cCGQ0d3uo9UfKByQ3sI6Audoy6dY&r=k0gMbcsdOcdbPUNV5tW66KQSZfXL0ewVDPVBp7tqbks&m=MY22nA7ZisOgCJYs2kzmSYVV3omhecjZxITaH8QoAZI&s=92rhDVQ2xQADAGVmFcqEh0UK0cUeH5inajmREsF0WEs&e=> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
