CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Alex Lee
Gesendet: Freitag, 5. Oktober 2018 05:37
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] [Offtopic] Protein stain in Coomassie Blue dyes
Hi All,
Thanks for all of your inputs!
Alex
On Thu, Oct 4, 2018 at 7:31 PM Zhijie Li
mailto:z
Hi Alex,
the binding of Coomassie Brilliant Blue R to proteins roughly correlates
with the number of positive charges (about 1.5-3 dye molecules/charge;
JBC 216, 9976-9980) whereas the SDS is thought to bind/align to the
protein backbone during/after heat denaturation (roughly 1 SDS per 2
Hi All,
Thanks for all of your inputs!
Alex
On Thu, Oct 4, 2018 at 7:31 PM Zhijie Li wrote:
> Hi,
>
> At high concentration (1-2%) the published saturating SDS:protein binding
> ratio is about 1.4:1 by weight, that is roughly one SDS molecule per two aa
> on average. It is dense but not that
Hi,
At high concentration (1-2%) the published saturating SDS:protein binding ratio
is about 1.4:1 by weight, that is roughly one SDS molecule per two aa on
average. It is dense but not that dense to prevent any further interaction.
More importantly, as a quite hydrophilic small molecule SDS
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Date: Thursday, October 4, 2018 at 8:24 PM
To: "CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>"
mailto:CCP4BB@JISCMAIL.AC.UK>>
Su
Dear All,
I am thinking that in an SDS-PAGE experiment, if protein samples are boiled
in SDS containing loading dye, and supposedly SDS interacts with proteins,
why the Coomassie Blue dyes could still interact with and stain the
proteins? I am thinking SDS is covering the proteins, making no
