I have 12 months BBSRC-funded support available for a PDRA in Leicester. This might suit someone who has still to finish writing up or being examined for their PhD, I am happy to discuss some flexibility. The post is available from 1 January until the end of December 2022. The research (in collaboration with the Co-I Prof Emma Raven, now at the Chemistry Department of the University of Bristol) is to understand heme peroxidase enzyme mechanism. For this we need to trap intermediates and determine the structures. There is controversy over the nature of these intermediates and we have used tools such as neutron crystallography and X-ray free electron lasers in the past which have resulted in high-impact publications. Specific remaining aims of this project include the direct determination of the protonation state of the Compound II intermediate of cytochrome c peroxidase using neutron crystallography and of the myoglobin analogue using neutron and XFEL techniques. We are also developing techniques for using microcrystal electron diffraction to study enzyme intermediates. We are interested in using a wide variety of biochemical and biophysical techniques to complement and verify the studies. This includes UV-Visible and X-ray spectroscopy. The group ( https://le.ac.uk/liscb/research-groups/peter-moody) is based in the Leicester Institute for Structural and Chemical Biology ( https://le.ac.uk/liscb ), a supportive and collegiate environment with access to world-class facilities and expertise. This post is suitable for someone interested in expanding their experience of, and pushing the boundaries of, methods to understand enzyme mechanisms. You will be competent working with proteins in the laboratory and want to learn about and apply physical and chemical techniques to biological problems
Please contact me using my university email, peter.moody @ le.ac.uk (without the spaces), do not reply to this email directly. These papers are some recent examples of our work: 1. H Kwon, J Basran, C Pathak, M Hussain, SL Freeman, AJ Fielding, AJ Bailey, N Stefanou, HA Sparkes, T Tosha, K Yamashita, K Hirata, H Murakami, G Ueno, H Ago, K Tono, M Yamamoto, H Sawai, Y Shiro, H Sugimoto, E Raven & PCE Moody (2021) XFEL Crystal Structures of Peroxidase Compound II XFEL Crystal Structures of Peroxidase Compound II. Angewandte Chemie Int. Edition doi: 10.1002/anie.202103010 2. H Kwon, J Basran, CM Casadei, AJ Fielding, TE Schrader, A Ostermann, JM Devos, P Aller, MP Blakeley, PCE Moody & EL Raven (2016) Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme Nature Communications 7, Article number: 13445 (2016) 3. H Kwon, J Basran, J M. Devos, R Suardiaz, MW van der Kamp, AJ Mulholland, TE Schrader, Andreas Ostermann, Matthew P. Blakeley, Peter C. E. Moody & Emma L. Raven (2020) Visualizing the protons in a metalloenzyme electron proton transfer pathway. Proc. Natl. Acad Sci. (USA) 117 (12) 6484-6490; 4. Hanna Kwon, Tobias E. Schrader, Andreas Ostermann, Matthew P. Blakeley, Emma L. Raven & Peter C.E. Moody (2020) Heme peroxidase—Trapping intermediates by cryo neutron crystallography Methods in Enzymology Vol 634 (Ed. P C E Moody) 5. H Kwon, PS Langan, L Coates, EL Raven & PCE Moody (2018) The rise of neutron cryo-crystallography. Acta Cryst. D74, 792-799 6. H Kwon, O Smith, EL Raven & PCE Moody (2017) Combining X-ray and Neutron Crystallography with Spectroscopy. Acta Cryst D72 141- 147 7. DD Turner, L Lad, H Kwon, J Basran, KH Carr, PCE Moody & EL Raven (2017) The role of Ala134 in controlling substrate binding and reactivity in ascorbate peroxidase J. Inorg. Biochem. Volume 180, March 2018, Pages 230-234 8. PCE Moody & EL Raven (2018) The Nature & Reactivity of Ferryl Heme in Compounds I and II Acc. Chem Res. 51, 2, 427–435 8. PCE Moody & EL Raven (2018) The Nature & Reactivity of Ferryl Heme in Compounds I and II Acc. Chem Res. 51, 2, 427–435 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
