Dear Jinjin Zhang,
what happens if you treat your data as perfect twin? Maybe you do have a
twin after all.
Best regards, Daniel
--
Daniel Schlieper email: [EMAIL PROTECTED]
Molecular Motors Group phone: +44 1883 722306 (x 305)
Marie Curie Research Institute
Dear Jinjin Zhang --
On 29 Jul 2008, at 22:34, JINJIN ZHANG wrote:
1. Protein 100 aa, peptide 6 aa, co-crystalized
2. Space group: P43212. Overall R-fac: 0.03. Redundancy: >5 for 98%
of reflections. B-factor:55
3. CNS refinement
4. Phenix xtriage checked, no twinning is suspected.
5. Water mo
How are you deciding your resolution limit? I/sigma, Rmrgd-F, Rmeas, etc.
etc.? We recently had a person in a fellow lab that couldn't figure why
their R/Rfree was mid- to high-twenties for their 1.8 angstrom structure.
Turned out the data stopped at 2.1.
Stuart
On Tuesday 29 July 2008 17:3
Dear All,
Here are updated details for the high R problem of my 1.75A structure.
1. Protein 100 aa, peptide 6 aa, co-crystalized
2. Space group: P43212. Overall R-fac: 0.03. Redundancy: >5 for 98% of
reflections. B-factor:55
3. CNS refinement
4. Phenix xtriage checked, no twinning is suspecte
(office); 508 856 1211 (lab)
e-mail: [EMAIL PROTECTED]
http://www.umassmed.edu/pmm/faculty/vandenberg.cfm
From: CCP4 bulletin board on behalf of JINJIN ZHANG
Sent: Tue 7/29/2008 5:24 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] 27.5% R/29% Rfree for 1.75A structure
Dear All,
Here are updated details for the high R problem of my 1.75A structure.
1. Protein 100 aa, peptide 6 aa, co-crystalized
2. Space group: P43212. Overall R-fac: 0.3. Redundancy: >5 for 98% of
reflections. B-factor:55
3. CNS refinement
4. Phenix xtriage checked, no twinning is suspected
