Hello, have you tried anisotropic B-factor refinement? It is usually very good
at cleaning-up the difference map. At that sort of resolution, you may still
have some way to go in the refinement since the R and R-free usually go to
about half of what you have, at least with proteins.
Best
Sometimes ghost like this mean there is a spacegroup error - absences can
be the result of the non-crystallographic translation and not be truly
indicitive of the spacegroup.
what is the possible spacegroup and what is the NC translation vector?
Eleanor
On Wed, 29 Jul 2020 at 14:13, Schreuder,
Dear Rafal,
At this resolution, one sees many amino-acid side chains with alternative
conformation, so it might be a good idea to test if this is also true for
nucleotides.
Best, Herman
-Ursprüngliche Nachricht-
Von: CCP4 bulletin board Im Auftrag von Rafal Dolot
Gesendet: Mittwoch,
