Re: [ccp4bb] AW: [ccp4bb] Cleaved peptide density!

2015-04-22 Thread CCP4
Once thing I don't fully understand: do you have a cleaved density or a cleaved peptide? If your peptide is sitting in a binding site, and that you can see only the part inside the pocket, with the rest supposably sitting in a 'more opened' part of the molecule, or else outside of the molecule,

[ccp4bb] AW: [ccp4bb] Cleaved peptide density!

2015-04-21 Thread Herman . Schreuder
Dear Dipankar, as Bonsor mentioned, 2-3 weeks is something completely different from the few hours normally used for enzyme assays. A lot may happen during the long crystallization that does not happen in the assay. Also, your enzyme will still have the remainder of the catalytic machinery