[ccp4bb] AW: [ccp4bb] Weired Crystal packing

2014-07-15 Thread Herman . Schreuder 
Dear Appu,

What is your problem? To me this crystal packing looks completely normal. If 
you look for tetramers in space groups with 422 symmetry I am sure you will 
find examples with a similar packing.

Best,
Herman


Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Appu 
kumar
Gesendet: Dienstag, 15. Juli 2014 00:57
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Weired Crystal packing

Dear All,
  I need your valuable suggestion in defining the arrangement 
of tetrameric protein in crystal upon symmetry mates information. I have 
attached a ppt for your evaluation. The monomer protein is composed of two 
domain (Domain 1 and Domain 2). In space group I422, the arrangement of 
tetramer upon symmetry mate information look weired because there are two kind 
of crystal contacts. The tetramer1 form crystal contact with tetramer2 through 
domain1 but on other side tetramer1 form crystal contact with tetramer3 through 
domain 2. Things will be clear if you will have look at the attached ppt. 
Therefore I request you to please look at the attached ppt.

I have looked at the crystal packing in many tetrameric proteins which are 
either arranged in head to head or tail to tail in crystal but not in both head 
to head and tail to tail contacts in same crystal. Is this kind of crystal 
packing is possible?

I need your valuable suggestion. Crystal is solved as monomer which upon 
symmetry mate information gives correct tetramer.

Re: [ccp4bb] AW: [ccp4bb] Weired Crystal packing

2014-07-15 Thread Eleanor Dodson 
I suggest submitting your coordinates to the PDBe (EBI) web site to run
PISA or using it via tthe CCP4I interface.
This analysing contacts, and suggests the most favoured packing. It  is not
fool proof but worth checking.
Eleanor



On 15 July 2014 09:53, herman.schreu...@sanofi.com wrote:

  Dear Appu,



 What is your problem? To me this crystal packing looks completely normal.
 If you look for tetramers in space groups with 422 symmetry I am sure you
 will find examples with a similar packing.



 Best,

 Herman





 *Von:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *Im Auftrag von
 *Appu kumar
 *Gesendet:* Dienstag, 15. Juli 2014 00:57
 *An:* CCP4BB@JISCMAIL.AC.UK
 *Betreff:* [ccp4bb] Weired Crystal packing



 Dear All,

   I need your valuable suggestion in defining the
 arrangement of tetrameric protein in crystal upon symmetry mates
 information. I have attached a ppt for your evaluation. The monomer protein
 is composed of two domain (Domain 1 and Domain 2). In space group I422, the
 arrangement of tetramer upon symmetry mate information look weired because
 there are two kind of crystal contacts. The tetramer1 form crystal contact
 with tetramer2 through domain1 but on other side tetramer1 form crystal
 contact with tetramer3 through domain 2. Things will be clear if you will
 have look at the attached ppt. Therefore I request you to please look at
 the attached ppt.



 I have looked at the crystal packing in many tetrameric proteins which are
 either arranged in head to head or tail to tail in crystal but not in both
 head to head and tail to tail contacts in same crystal. Is this kind of
 crystal packing is possible?



 I need your valuable suggestion. Crystal is solved as monomer which upon
 symmetry mate information gives correct tetramer.