Pete
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf
Of Jim Pflugrath
Sent: Tuesday, April 29, 2008 4:24 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Are Calcium Citrate Crystals A Common False
Positive?
That's an easy hypothesis to test.
etin board [mailto:[EMAIL PROTECTED] On Behalf Of Jim Pflugrath
Sent: Tuesday, April 29, 2008 4:24 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Are Calcium Citrate Crystals A Common False Positive?
That's an easy hypothesis to test. Simply set up your drops with the same
conditions
Jim Pflugrath wrote:
> That's an easy hypothesis to test. Simply set up your drops with the
> same conditions except without protein and see if you get crystals.
Not always. I've had a few cases where the protein turns out to be the
essential additive in order to obtain salt crystals!
Looking
That's an easy hypothesis to test. Simply set up your drops with the same
conditions except without protein and see if you get crystals. Please let
us know the results. Thanks!
Jim
On Tue, 29 Apr 2008, Sam Stephenson wrote:
Are calcium citrate crystals a common false positive in trays with
Are calcium citrate crystals a common false positive in trays with up to 200mM
of each? There is absolutely no phosphate in the trays so I'm almost positive
they're not calcium phosphate.
Cheers,
Sam
-
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