Re: [ccp4bb] Biacore/SPR
As Juergen has mentioned, both the T200 and the BioRad ProteOn (XPR36) are good options (we have both). The XPR36 is a higher throughput machine and can also be used for small molecule work (not quite as sensitive as the T200, but more sensitive than we had anticipated and certainly higher throughput). Good luck, tom Tom Peat Biophysics Group CSIRO, CMSE 343 Royal Parade Parkville, VIC, 3052 +613 9662 7304 +614 57 539 419 tom.p...@csiro.au From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Bosch, Juergen [jubo...@jhsph.edu] Sent: Tuesday, October 29, 2013 2:05 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Biacore/SPR BiaCore 3000 or if you can afford the T200. Proteon XPR36 would be a cheaper Option and should work as well. Jürgen On Oct 28, 2013, at 10:54 AM, Gang Dong wrote: We mainly measure protein-protein interactions (sometimes protein-small molecules). Thanks! _Gang From: Bosch, Juergen [mailto:jubo...@jhsph.edu] Sent: Monday, October 28, 2013 3:17 PM To: Gang Dong Cc: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] Biacore/SPR Protein-protein interaction? protein-small molecule interactions ? Epitope mapping of mABs ? Could you specify what you would like to do, as different models are good for different things. Jürgen On Oct 28, 2013, at 10:08 AM, Gang Dong wrote: Dear all, Could anyone tell me your experience with Biacore (any models) or a similar SPR-based technology for measuring interaction kinetics? I also want to know the price ranges to discuss with our department/facility managers. Thanks, Gang .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
Re: [ccp4bb] Biacore/SPR
Another option is BLItz which is cheaper and uses interferometry. Dee Date: Mon, 28 Oct 2013 11:05:33 -0400 From: jubo...@jhsph.edu Subject: Re: [ccp4bb] Biacore/SPR To: CCP4BB@JISCMAIL.AC.UK BiaCore 3000 or if you can afford the T200.Proteon XPR36 would be a cheaper Option and should work as well.Jürgen On Oct 28, 2013, at 10:54 AM, Gang Dong wrote:We mainly measure protein-protein interactions (sometimes protein-small molecules). Thanks! _Gang From: Bosch, Juergen [mailto:jubo...@jhsph.edu] Sent: Monday, October 28, 2013 3:17 PM To: Gang Dong Cc: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Biacore/SPR Protein-protein interaction?protein-small molecule interactions ?Epitope mapping of mABs ?Could you specify what you would like to do, as different models are good for different things.Jürgen On Oct 28, 2013, at 10:08 AM, Gang Dong wrote: Dear all, Could anyone tell me your experience with Biacore (any models) or a similar SPR-based technology for measuring interaction kinetics? I also want to know the price ranges to discuss with our department/facility managers. Thanks,Gang .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
Re: [ccp4bb] Biacore / SPR
Dear Dong, Biacore T200 is the best choice for protein/small molecule interaction analysis. the Biacore 3000 works perfectly for protein-protein interactions. hope that help you. Badr -- Dr Douzi Badreddine Architecture and Function of Biological Macromolecules (AFMB), CNRS UMR7257 163 Avenue de Luminy, Case 932-13009 Marseille, France. Tel: +33 4 91 85 55 93 Fax: +33 4 9126 67 20 E-mail : badreddine.do...@afmb.univ-mrs.fr
Re: [ccp4bb] Biacore/SPR
BiaCore 3000 or if you can afford the T200. Proteon XPR36 would be a cheaper Option and should work as well. Jürgen On Oct 28, 2013, at 10:54 AM, Gang Dong wrote: We mainly measure protein-protein interactions (sometimes protein-small molecules). Thanks! _Gang From: Bosch, Juergen [mailto:jubo...@jhsph.edu] Sent: Monday, October 28, 2013 3:17 PM To: Gang Dong Cc: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: Re: [ccp4bb] Biacore/SPR Protein-protein interaction? protein-small molecule interactions ? Epitope mapping of mABs ? Could you specify what you would like to do, as different models are good for different things. Jürgen On Oct 28, 2013, at 10:08 AM, Gang Dong wrote: Dear all, Could anyone tell me your experience with Biacore (any models) or a similar SPR-based technology for measuring interaction kinetics? I also want to know the price ranges to discuss with our department/facility managers. Thanks, Gang .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
Re: [ccp4bb] Biacore/SPR
We mainly measure protein-protein interactions (sometimes protein-small molecules). Thanks! _Gang From: Bosch, Juergen [mailto:jubo...@jhsph.edu] Sent: Monday, October 28, 2013 3:17 PM To: Gang Dong Cc: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Biacore/SPR Protein-protein interaction? protein-small molecule interactions ? Epitope mapping of mABs ? Could you specify what you would like to do, as different models are good for different things. Jürgen On Oct 28, 2013, at 10:08 AM, Gang Dong wrote: Dear all, Could anyone tell me your experience with Biacore (any models) or a similar SPR-based technology for measuring interaction kinetics? I also want to know the price ranges to discuss with our department/facility managers. Thanks, Gang .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
Re: [ccp4bb] Biacore/SPR
Protein-protein interaction? protein-small molecule interactions ? Epitope mapping of mABs ? Could you specify what you would like to do, as different models are good for different things. Jürgen On Oct 28, 2013, at 10:08 AM, Gang Dong wrote: Dear all, Could anyone tell me your experience with Biacore (any models) or a similar SPR-based technology for measuring interaction kinetics? I also want to know the price ranges to discuss with our department/facility managers. Thanks, Gang .. Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
[ccp4bb] Biacore/SPR
Dear all, Could anyone tell me your experience with Biacore (any models) or a similar SPR-based technology for measuring interaction kinetics? I also want to know the price ranges to discuss with our department/facility managers. Thanks, Gang
