[ccp4bb] Biorad Profinia vs GE AKTAPrime Plus, for affinity tagged proteins
Dear all, My lab is looking into buying a protein purification system. The AKTA is more versatile, but does anyone know if the Profinia is in any way superior to the AKTA for purification of affinity-tagged proteins? If it is, I would really appreciate if you can tell me in what way it is superior. Thanks, Sangeetha.
Re: [ccp4bb] Biorad Profinia vs GE AKTAPrime Plus, for affinity tagged proteins
Thanks for your response, Edward. Yes, that is what I thought. For the most part, people plan to tag their proteins in my lab. I'll possibly be the only one purifying non-tagged proteins in my lab. I love the AKTA. Another was all praise of the Profinia. To my knowledge, tagged proteins don't always get fully purified with just an affinity column. An additional size exclusion step helps. That is, if the tag is even available at the surface for binding the affinity column. My vote is AKTA. So, I wanted to find out what the world thought about both. On Thu, Jan 28, 2010 at 11:16 AM, Edward Collins edward_coll...@med.unc.edu wrote: personally, I don't know why you would want to limit yourself to just using affinity tags to purify your proteins. For my money the flexibility of the AKTA is critical. good luck -ed On Jan 28, 2010, at 11:08 AM, Sangeetha Vedula wrote: Dear all, My lab is looking into buying a protein purification system. The AKTA is more versatile, but does anyone know if the Profinia is in any way superior to the AKTA for purification of affinity-tagged proteins? If it is, I would really appreciate if you can tell me in what way it is superior. Thanks, Sangeetha.
Re: [ccp4bb] Biorad Profinia vs GE AKTAPrime Plus, for affinity tagged proteins
You really don't need much of a system to do just affinity purification, so almost anything would be workable unless you are doing really high throughput work. On the other hand, if you need to do traditional protein purification (IEX, HIC, GEC), which would include untagged proteins, and polishing of tagged or untagged proteins, then a good chromatography system is a must. I've owned, used, and abused an AKTA FPLC since 1999. I think I have one of the first units. It is built like a tank, and is still expandable. We just upgraded the computer system and associated hardware to a modern OS (Win NT4 to XP). I don't know about the Profina units, but the AKTA system can take daily abuse with aplomb. I't been in a cold room for over 10 years and it's still going. Cheers. On 1/28/2010 11:08 AM, Sangeetha Vedula wrote: Dear all, My lab is looking into buying a protein purification system. The AKTA is more versatile, but does anyone know if the Profinia is in any way superior to the AKTA for purification of affinity-tagged proteins? If it is, I would really appreciate if you can tell me in what way it is superior. Thanks, Sangeetha. -- Roger S. Rowlett Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu
Re: [ccp4bb] Biorad Profinia vs GE AKTAPrime Plus, for affinity tagged proteins
We've been using Bio-Rad biologic duoflow FPLCs for over 5 years (we have 4 of them on site). They can also handle abuse and cold room storage. Their hardware and software are user-friendly. It seems the Profinia system is geared toward simpler purifications.
