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Caroline Larabell Professor, University of California San Francisco Faculty Scientist, Lawrence Berkeley National Laboratory Wednesday, July 19, 2023, 13:30 EDT (New York time) Soft X-ray Tomography: Quantitative Structural Cell Biology at the Mesoscale https://bnl.zoomgov.com/meeting/register/vJItdeyrrTkoGfb4oMXZMF9r_J58cZ_Dfd0 Abstract Soft X-ray tomography (SXT) is similar in concept to the well-established medical diagnostic technique, computed axial tomography (CAT), except SXT is capable of imaging single cells up to 20 µm diameter with a spatial resolution of 35 nm or better. Cells are simply rapidly frozen, placed in a goniometer, and imaged through 360 degrees to achieve isotropic resolution. Cells imaged by SXT are, therefore, highly representative of the cell in its native, functional state. Cells are illuminated with x-ray photons from within a region of the spectrum known as the 'water window' (284 – 543eV). 'Water window' x-ray photons are absorbed an order of magnitude more strongly by carbon- and nitrogen-containing organic material than by water. Consequently, variation in biomolecule composition and concentration gives rise to quantitative, high-contrast images of intact, fully hydrated cells without the need to use contrast-enhancing agents. Attenuation of soft x-rays as they pass through the specimen adheres to the Beer-Lambert Law, yielding unique quantitative Linear Absorption Coefficient (LAC) measurements for cellular components. Three-dimensional images of an entire cell can be obtained in about ten minutes, enabling analyses of large number of cells with multiple manipulations. At the National Center for X-ray Tomography in Berkeley California, we are working with a large number of scientists from around the world to image a variety of different cell types. I will present highlights of some of these projects to demonstrate the unique information that can be generated by SXT and the broad spectrum of biological applications. =============================================================================== Vivian Stojanoff, PhD Education, Training, Outreach User Program p 1(631) 344 8375 e nsls.lifescien...@gmail.com w https://www.bnl.gov/ps/lifesciences/<https://www.bnl.gov/ps/lsbr/> Address: Center for Biomolecular Structure National Synchrotron Light Source II Building 745 Brookhaven National Laboratory Upton NY 11973 Supporting Grants: CBMS is supported by NIH-NIGMS #P30GM133893, and by the DOE-BER #KP1605010. Any work performed at NSLS-II is supported by DOE-BES under contract # DE-SC0012704. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
