Dear all, the next MX-proposal application deadline: September 01, 2020 is approaching
As usual, all proposals will be handled by our electronic user portal GATE, https://www.helmholtz-berlin.de/pubbin/hzbgate Hereby we would like to invite the submission of new proposals for MX-beamtime at the HZB-MX beamlines for the next beam time period (03/2021-08/2021). In order to apply for beamtime, please register in GATE and submit a new beam time application proposal. Please note that we now expect from each research group only ONE proposal, which can contain up to 20 individual projects. IMPORTANT: If you have a running 2019-1 or a running 2020-1 proposal, you may ask for extension. For a 2020-1 proposal, an interim report is necessary, and for a 2019-1 proposal a full report including highlights. You will also be able to edit and modify your proposals by adding and deleting projects. HZB provides MX-beamtime at the three MX-beamlines BL14.1, BL14.2 and BL14.3. The three beamlines are equipped with state-of-the-art instrumentation and are currently the most productive MX-stations in Germany with more than 3300 PDB depositions in total. Beamtime is granted based on the reviewed proposals and on reports from previous research activities. Please make sure to include them if available. Experimental setup: BL14.1: - Photon energy range: 5.5-16 keV (wavelength: 0.775-2.25 A) - Photon flux: 1.8x10¹¹ Phot/sec x 100 mA at sample position (0.04-1 sec exposure time per frame) - PILATUS3 S 6M detector with 1000 µm Si sensor thickness, 141 mm-680 mm max. distance from the sample - Microdiffractometer (MD2) with Mini-kappa goniometer - Automatic sample changer (CATS), 90 sample storage capacity (SPINE-Pin & EMBL sample magazine compatibility) IMPORTANT: this will be upgraded to UNIPUCKS only in September 2020 - User defined beam shaping from 50 µm-100 µm diameter possible - In situ crystal-screening using 96-well plates - 32-core XEON-CPU server, with 10GB uplink to Pilatus 6M - Data collection control via MXCuBE2 - Common MX-software installed including EDNA, XDS, iMOSFLM, CCP4, Phenix, SHELXC-D-E, etc. - Automated data processing using XDSAPP - Remotely controlled cryo-shutter for crystal annealing - AMPTEK-XRF detector and XFEPLOT software available We are also offering the hard- and software environment for carrying out UV-RIP experiments at BL14.1. For further information, please visit: https://www.helmholtz-berlin.de/forschung/oe/np/gmx/ancillary-facilities/uvrip_en.html BL14.2: - Photon energy range: 5.5-16 keV (wavelength: 0.775-2.25 A) - Photon flux: 1.6x10¹¹ Phot/sec x 100 mA at sample position (0.05-1 sec exposure time per frame) - PILATUS3 S 2M detector with 1000 µm Si sensor thickness, 85 mm - 800 mm distance from the sample (a special mode with 56 mm distance is also available upon request) - Nanodiffractometer with fast air-bearing axis and on-axis sample microscope - User defined beam shaping from 30 µm-150 µm diameter possible - Data collection control via MXCuBE2 - G-ROB sample changer for SPINE and UNIPUCK support - 60-core XEON-CPU server, with fibre channel SAN up-link data processing environment - Common MX-software installed including EDNA, XDS, iMOSFLM, CCP4, Phenix, SHELXC-D-E, etc. - Automated data processing using XDSAPP - AMPTEK-XRF detector and XFEPLOT software available - UV-Microsprectrophotometer offline setup available If you need atomic resolution or better, BL14.2 is the beamline of choice for you!! BL14.3: - Fixed photon energy: 13.8 keV (wavelength: 0.89 A) - Photon flux: 1.6x10exp10 Phot/sec x 100mA at sample position (3-20 sec exposure time per frame) - PILATUS 6M detector, 54 mm-450 mm distance from the sample - MD2S microdiffractometer with mini-kappa goniometer - In situ crystal-screening using 96-well plates - RT data collection - 60-core XEON-CPU server, with fibre channel SAN up-link data processing environment - Data collection control via MXCuBE2 - Common MX software installed including EDNA, XDS, iMOSFLM, CCP4, Phenix, SHELXC-D-E, etc. - Automated data processing using XDSAPP - Remotely controlled cryo-shutter for crystal annealing - REX rapid nozzle exchanger - HC-Lab dehydration device installed (please specify HC-Lab-beamtime in your proposal if needed) - AMPTEK-XRF detector and XFEPLOT software available Other facilities: - Ultra high performance stereo microscope Leica M205A, 20-255x zoom, 8 Mpix CCD-camera - Pressure chamber for noble gas derivatization (Xe, Kr available upon request) S1-biolab facilities (separate registration required): - Protein production and purification (AEKTA) - nL 96-well crystallization plate formulation and storage at 5°C and 20°C - Biophysical characterization with real time PCR (thermofluor assay) - Contactless compound pipetting using ATS The HZB-MX group is also providing expert assistance as well as access to a library of fragments for carrying out crystallographic fragment-screening experiments. For more information please see https://www.helmholtz-berlin.de/forschung/oe/np/gmx/fragment-screening/index_en.html or contact mswe...@helmholtz-berlin.de<mailto:mswe...@helmholtz-berlin.de>. Support for fragment-screening experiments is also possible via the EU-project iNEXT-Discovery. For this, applications have to be submitted through the iNEXT-Discovery web site. Please visit our web page www.helmholtz-berlin.de/bessy-mx<http://www.helmholtz-berlin.de/bessy-mx> in order to obtain updated information about our experimental setup and other requirements. Manfred Weiss and the HZB-MX team -- Dr. Manfred S. Weiss Macromolecular Crystallography Helmholtz-Zentrum Berlin Albert-Einstein-Str. 15 D-12489 Berlin Germany ________________________________ Helmholtz-Zentrum Berlin für Materialien und Energie GmbH Mitglied der Hermann von Helmholtz-Gemeinschaft Deutscher Forschungszentren e.V. Aufsichtsrat: Vorsitzender Dr. Volkmar Dietz, stv. Vorsitzende Dr. Jutta Koch-Unterseher Geschäftsführung: Prof. Dr. Bernd Rech (Sprecher), Prof. Dr. Jan Lüning, Thomas Frederking Sitz Berlin, AG Charlottenburg, 89 HRB 5583 Postadresse: Hahn-Meitner-Platz 1 D-14109 Berlin ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
