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Dear Mike,
I'd be surprised if the book was from Hampton: The pH-value of Hampton
solutions refers to the stock solution of the buffer before setting up
the final solution, and can differ a lot from your drop's pH - e.g. in
the presence of Imidazole
That may be the CALBIOCHEM Buffers Booklet, which is free online as a PDF.
On 01/17/2013 12:38 AM, Mike John wrote:
Hello,
Shameful and sorry for asking this simple question, it looks like this
when first starting a new setup in
so-called structural biology.
I remmeber a book of, probably,
Hello,
Shameful and sorry for asking this simple question, it looks like this when
first starting a new setup in
so-called structural biology.
I remmeber a book of, probably, Hampton, in which there are tables of pH
optimization for many buffers. For example for buffer TRIS, the table will
Hi Mike,
check out the EZ screen builder from Emerald Bio, that is a really nice tool
for this purpose: http://www.emeraldbiosystems.com/escreentoolkit/escreen.html
Select your screen, your hit condition, then hit 'optimize' and you will have a
screen that will vary the precipitant
