Re: [ccp4bb] DNA or RNA
I think you could use Qubit. https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit.html On Thu, Jun 20, 2019 at 5:17 PM Reza Khayat wrote: > Hi, > > > Sorry for the non-crystallography question. We have a protein complex with > nucleic acid and would like to know if the nucleic acid is DNA or RNA. Is > there a sensitive method for doing this where we don't need buckets of the > sample? Thanks. > > > Best wishes, > Reza > > Reza Khayat, PhD > Assistant Professor > City College of New York > Department of Chemistry > New York, NY 10031 > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
Re: [ccp4bb] DNA or RNA
Hi Reza, I'm not sure if this is sensitive enough, but Stains-All might be worth a try. Cationic carbocyanine dye used for differential staining of nuclei acids and proteins with RNA staining blue/purple, DNA staining blue, and proteins staining red. https://www.abcam.com/stains-all-cationic-carbocyanine-dye-ab146278.html Aaron On Thu, Jun 20, 2019 at 3:17 PM Reza Khayat wrote: > Hi, > > > Sorry for the non-crystallography question. We have a protein complex with > nucleic acid and would like to know if the nucleic acid is DNA or RNA. Is > there a sensitive method for doing this where we don't need buckets of the > sample? Thanks. > > > Best wishes, > Reza > > Reza Khayat, PhD > Assistant Professor > City College of New York > Department of Chemistry > New York, NY 10031 > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
Re: [ccp4bb] DNA or RNA
Hi Reza, What about seeing whether RNAse and DNAse incubations kill the complex? JPK + Jacob Pearson Keller Research Scientist / Looger Lab HHMI Janelia Research Campus 19700 Helix Dr, Ashburn, VA 20147 Desk: (571)209-4000 x3159 Cell: (301)592-7004 + The content of this email is confidential and intended for the recipient specified in message only. It is strictly forbidden to share any part of this message with any third party, without a written consent of the sender. If you received this message by mistake, please reply to this message and follow with its deletion, so that we can ensure such a mistake does not occur in the future. From: CCP4 bulletin board On Behalf Of Reza Khayat Sent: Thursday, June 20, 2019 6:16 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] DNA or RNA Hi, Sorry for the non-crystallography question. We have a protein complex with nucleic acid and would like to know if the nucleic acid is DNA or RNA. Is there a sensitive method for doing this where we don't need buckets of the sample? Thanks. Best wishes, Reza Reza Khayat, PhD Assistant Professor City College of New York Department of Chemistry New York, NY 10031 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1=DwMFAw=LU6cRtx0xgB8s29tIz9Olw=eLCg9eJ4Rs_LnxfUWsp7FSxhIEcZYmTSU4Uyq1bRYPI=SRjjlDfYGt6rV7_06BxoOeHWYMb6orFgBbuzVpy0r7g=kAFc_Gfsb6lQoHMaWIzpVd_0l0XJxDCP9dViMfF-vFE=> To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] DNA or RNA
Hi, Sorry for the non-crystallography question. We have a protein complex with nucleic acid and would like to know if the nucleic acid is DNA or RNA. Is there a sensitive method for doing this where we don't need buckets of the sample? Thanks. Best wishes, Reza ? Reza Khayat, PhD Assistant Professor City College of New York Department of Chemistry New York, NY 10031 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
