Re: [ccp4bb] Direct crystallization of Lysozyme from eggs

2012-09-25 Thread Jacob Keller
Alhtough perhaps a little more macabre--maybe you could crystallize
hemoglobin or myoglobin from blood or muscle (meat?). I recollect some
story of whale myo- or hemo-globin crystallizing on the salty decks of a
whaling ship...

Jacob



On Tue, Sep 25, 2012 at 11:59 AM, David Smith  wrote:

> Dear crystallographers,
>
> I am working on an outreach program through the APS to get motivated high
> school students some/more research experience.  As I work at a
> crystallography beamline (LS-CAT), we are trying to get a
> crystallographically centered experiment in place that will be interesting
> to a high school student.  Among other ideas, I had mentioned to the
> teacher the possibility of crystallizing lysozyme directly from egg
> whites.  The teacher picked up on this idea as the one he and his students
> would like to pursue.  I have done a bit of reseach and I have found the
> Alderton and Fevold paper from '46 about direct crystallization from egg
> whites.  However I am unable to find any papers that refine or expand upon
> this experiment.  While there appears to be some literature about lysozyme
> and crystallizing the same, these papers do not use lysozyme directly from
> hen egg whites.  Do any of you know of any more recent papers or procedures
> that would be relevant or easily adapted to an high school environment?
>
> Our goal is to get diffraction quality crystals, expose the crystals at
> the beamline, and present a poster of the experience.
>
> Cheers and thanks,
>
> David Smith
>
> --
> David W Smith
> Research Scientist
> LS-CAT
> APS, Argonne IL
> W:(630)343-9811
> F:(630)252-4664
>



-- 
***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
email: j-kell...@northwestern.edu
***


[ccp4bb] Direct crystallization of Lysozyme from eggs

2012-09-25 Thread David Smith
Dear crystallographers,

I am working on an outreach program through the APS to get motivated high
school students some/more research experience.  As I work at a
crystallography beamline (LS-CAT), we are trying to get a
crystallographically centered experiment in place that will be interesting
to a high school student.  Among other ideas, I had mentioned to the
teacher the possibility of crystallizing lysozyme directly from egg
whites.  The teacher picked up on this idea as the one he and his students
would like to pursue.  I have done a bit of reseach and I have found the
Alderton and Fevold paper from '46 about direct crystallization from egg
whites.  However I am unable to find any papers that refine or expand upon
this experiment.  While there appears to be some literature about lysozyme
and crystallizing the same, these papers do not use lysozyme directly from
hen egg whites.  Do any of you know of any more recent papers or procedures
that would be relevant or easily adapted to an high school environment?

Our goal is to get diffraction quality crystals, expose the crystals at the
beamline, and present a poster of the experience.

Cheers and thanks,

David Smith

-- 
David W Smith
Research Scientist
LS-CAT
APS, Argonne IL
W:(630)343-9811
F:(630)252-4664