What odds to you give us?
...I bet on disordered DTT covalently bound to the protein.
Mark J van Raaij
Lab 20B
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://www.cnb.csic.es/~mjvanraaij
On 19 Aug
Convent Avenue
New York, NY 10031
Tel. (212) 650-6070
www.khayatlab.org
Original message
Date: Tue, 19 Aug 2014 16:39:36 +0200
From: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK (on behalf of Mark J van
Raaij mjvanra...@cnb.csic.es)
Subject: Re: [ccp4bb] Enigmatic electron density attached
Hi Bernhard,
It is difficult guess with two dimensional images. Is it possible a metal
coordinated by Cys-Sulfur and one or two acetate ions?
HTH,
Partha
Sent from my iPhone
On Aug 19, 2014, at 10:12 AM, Bernhard Loll l...@chemie.fu-berlin.de wrote:
Dear all,
We are currently working
The planarity and symmetry rules out a mixed disulfide with DTT. Wrong
size/shape for glycerol. Without looking at your crystallization reagents,
I would have suggested something like an S-hydroxycysteine adduct of
something like 2,4-pentanedione or a dehydrated MPD alkene. Could
pentanedione be a
