Re: [ccp4bb] Eukaryotic Membrane Proteins
Hi there, The human protein FLAP from the MAPEG family was expressed in E. coli and also the K-channel, KirBac1.3 (although this is a chimera of mouse and B. xenovorans). The pubmed id codes for related papers are: 17703190 and 17600184 and the pdb codes for the structures are: 2QKS and 2Q7M. Hope this helps. Regards, Karen On 5 Mar 2009, at 13:04, Raji Edayathumangalam wrote: Hello Folks, My enquiry pertains to membrane proteins. If you have been able to successfully express a eukaryotic integral MEMBRANE protein in E. coli or know of such a case, would you please provide some details. Thanks. Raji Dr. Karen McLuskey Glasgow Biomedical Research Centre University of Glasgow G12 8TA Tel: +44 141 330 4476 -
Re: [ccp4bb] Eukaryotic Membrane Proteins
Hi Raji- A number of GPCRs have been overexpressed in E. coli. Examples are Reinhard Grishammer's work with neurotensin receptor and Christopher Tate's work with B2-adrenergic and adenosine 2a receptors. The problem was not necessarily expression but proper outer membrane targeting of *active* receptors. This seems to have been overcome for the most part by the use of either helpful lipid additives like cholesterol analogs or by brute-force mutagenesis to find more stable and active receptors. However, I don't know of any crystals or structures from any GPCR system generated from bacteria. Hope this helps... Brad On Thu, Mar 5, 2009 at 8:04 AM, Raji Edayathumangalam r...@brandeis.eduwrote: Hello Folks, My enquiry pertains to membrane proteins. If you have been able to successfully express a eukaryotic integral MEMBRANE protein in E. coli or know of such a case, would you please provide some details. Thanks. Raji
